Inositol Hexakisphosphate Promotes Dynamin I-Mediated Endocytosis

Membrane homeostasis is maintained by exocytosis and endocytosis. The molecular mechanisms regulating the interplay between these two processes are not clear. Inositol hexakisphosphate (InsP6) is under metabolic control and serves as a signal in the pancreatic β cell stimulus-secretion coupling by i...

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Published in:Proceedings of the National Academy of Sciences - PNAS 2002-05, Vol.99 (10), p.6773-6777
Main Authors: Høy, Marianne, Efanov, Alexander M., Bertorello, Alejandro M., Zaitsev, Sergei V., Olsen, Hervør L., Bokvist, Krister, Leibiger, Barbara, Leibiger, Ingo B., Zwiller, Jean, Berggren, Per-Olof, Gromada, Jesper
Format: Article
Language:English
Subjects:
Animals
Biological Sciences
Calcium - metabolism
Capacitance
Cellular biology
Dyes
Dynamin I
Dynamins
Electric Capacitance
Endocytosis
Endocytosis - drug effects
Endocytosis - physiology
Exocytosis
Fluorescence
GTP Phosphohydrolases - genetics
GTP Phosphohydrolases - metabolism
Inositols
Islet cells
Islets of Langerhans - metabolism
Islets of Langerhans - physiology
Medicin och hälsovetenskap
Membranes
Mice
Molecules
Phosphatases
Phosphatidylinositol 4,5-Diphosphate - metabolism
Phosphatidylinositol 4,5-Diphosphate - pharmacology
Phosphatidylinositols
Phosphorylation
Phytic Acid - metabolism
Phytic Acid - pharmacology
Studies
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Summary:Membrane homeostasis is maintained by exocytosis and endocytosis. The molecular mechanisms regulating the interplay between these two processes are not clear. Inositol hexakisphosphate (InsP6) is under metabolic control and serves as a signal in the pancreatic β cell stimulus-secretion coupling by increasing Ca2+-channel activity and insulin exocytosis. We now show that InsP6also promotes dynamin I-mediated endocytosis in the pancreatic β cell. This effect of InsP6depends on calcineurin-induced dephosphorylation and is accounted for by both activation of protein kinase C and inhibition of the phosphoinositide phosphatase synaptojanin and thereby formation of phosphatidylinositol 4,5-bisphosphate. In regulating both exocytosis and endocytosis, InsP6thus may have an essential integral role in membrane trafficking.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.102157499