Activation and Apoptosis of Murine Peritoneal Macrophages by Acute Cold Stress

• Published in: Biochemical and biophysical research communications 2001-05, Vol.283 (3), p.700-706
• Main Authors: Kizkai, Takako, Suzuki, Kenji, Hitomi, Yoshiaki, Iwabuchi, Kazuya, Onoé, Kazunori, Ishida, Hitoshi, Izawa, Tetsuya, Ji, Li Li, Ohno, Hideki
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; CD18 Antigens - metabolism; Cold Temperature - adverse effects; DNA fragmentation; Fgr; IL-1; In Vitro Techniques; Interleukin-1 - genetics; Interleukin-1 - metabolism; Lipopolysaccharides - toxicity; LPS; Macrophage Activation; Macrophage-1 Antigen - metabolism; Macrophages, Peritoneal - immunology; Macrophages, Peritoneal - pathology; Male; Medicin och hälsovetenskap; Mice; Mice, Inbred C57BL; Phagocytosis; Protein-Tyrosine Kinases - biosynthesis; Proto-Oncogene Proteins - biosynthesis; RNA, Messenger - genetics; RNA, Messenger - metabolism; src-Family Kinases; Stress, Physiological - immunology; Stress, Physiological - pathology; Time Factors
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.2001.4843

Effects of acute cold stress (5°C for 24 h) on the functions of peritoneal macrophages and the mechanisms for controlling host homeostasis were investigated in mice. Phagocytic activity and expression of the cell surface adhesion molecule CD11b/CD18 were markedly increased in peritoneal exudate cells by acute cold stress. These alterations were attributable to an increased number and phenotypical changes of adherent cells from acute cold-stressed mice. On the other hand, a lipopolysaccharide-induced activity of src-family tyrosine kinase Fgr, an expression of interleukin-1β (IL-1 β) mRNA, and a bioactivity of IL-1 in the culture supernatants of adherent cells from acute cold-stressed mice were markedly lower than those from control mice. A time course study revealed that the number of adherent cells in peritoneal exudate cells was markedly increased in mice exposed to cold for 24 h but returned to normal numbers when mice were exposed to cold for 72 h. DNA fragmentation and Annexin-V+ cells were observed in peritoneal exudate cells from acute-cold stressed mice. Thus, cold stress activated macrophages but these macrophages were destined to be eliminated by apoptosis.