Application of the use of high-throughput technologies to the determination of protein structures of bacterial and viral pathogens

• Published in: Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2006-10, Vol.62 (10), p.1196-1207
• Main Authors: Fogg, M. J., Bahar, M., Alzari, P., Bertini, I., Betton, J.-M., Burmeister, W. P., Cambillau, C., Coll, M., Canard, B., Carrondo, M., Daenke, S., Dym, O., Geerlof, A., Egloff, M.-P., Haouz, A., Enguita, F. J., Jones, T. A., Ma, Qingjun, Manicka, S. N., Owens, R.J., Migliardi, M., Nordlund, P., Peleg, Y., Schneider, G., Schnell, R., Stuart, D. I., Unge, T., Tarbouriech, N., Wilkinson, A. J., Wilmanns, M., Wilson, K. S., Zimhony, O., Grimes, J. M.
• Format: Article
• Language: English
• Subjects: Animals; Automation; Bacteria; Bacterial Infections; Bacterial Infections - metabolism; Bacterial Infections - microbiology; Bacterial Proteins; Bacterial Proteins - chemistry; Biochemistry, Molecular Biology; Bioinformatics; Biologi; Biological Physics; Biology; Cell and molecular biology; Cell- och molekylärbiologi; Cellular Biology; Chemical Sciences; Cloning; Computer Science; Cristallography; Expression; Humans; Life Sciences; Medicin och hälsovetenskap; Miniaturization; NATURAL SCIENCES; NATURVETENSKAP; Physics; Protein Folding; Proteomics; Proteomics - methods; Research Papers; Structural Biology; Viral Proteins; Viral Proteins - chemistry; Virus Diseases; Virus Diseases - metabolism; Virus Diseases - virology; Viruses
• ISSN: 1399-0047; 0907-4449; 2059-7983; 1399-0047; 2059-7983
• DOI: 10.1107/S0907444906030915

The Structural Proteomics In Europe (SPINE) programme is aimed at the development and implementation of high‐throughput technologies for the efficient structure determination of proteins of biomedical importance, such as those of bacterial and viral pathogens linked to human health. Despite the challenging nature of some of these targets, 175 novel pathogen protein structures (∼220 including complexes) have been determined to date. Here the impact of several technologies on the structural determination of proteins from human pathogens is illustrated with selected examples, including the parallel expression of multiple constructs, the use of standardized refolding protocols and optimized crystallization screens.