Tobacco smoke-associated N7-alkylguanine in DNA of larynx tissue and leucocytes

The presence of N7-alkylguanine adducts in DNA was analysed in a group of 46 patients with larynx tumours. All patients were subjected to laryngectomy and the tissues accessible for analysis by 32P-post-labelling assay were larynx tumour, larynx non-tumour and peripheral blood leucocytes. N7-Alkylgu...

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Bibliographic Details
Published in:Carcinogenesis (New York) 1996-03, Vol.17 (3), p.501-506
Main Authors: Szyfter, K., Hemminki, K., Szyfter, W., Szmeja, Z., Banaszewski, J., Pabiszczak, M.
Format: Article
Language:English
Subjects:
Adult
Aged
Alkylating Agents - analysis
Biological and medical sciences
DNA Adducts - analysis
Female
Guanine - analogs & derivatives
Guanine - analysis
Humans
Laryngeal Neoplasms - chemistry
Larynx - chemistry
Leukocytes - chemistry
Male
Medical sciences
Medicin och hälsovetenskap
Middle Aged
Nicotiana - chemistry
Plants, Toxic
Pneumology
Smoke
Tumors of the respiratory system and mediastinum
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Summary:The presence of N7-alkylguanine adducts in DNA was analysed in a group of 46 patients with larynx tumours. All patients were subjected to laryngectomy and the tissues accessible for analysis by 32P-post-labelling assay were larynx tumour, larynx non-tumour and peripheral blood leucocytes. N7-Alkylguanine adducts were detected in all the studied DNA samples. The average level of N7-alkylguanines was 26.2/107 nucleotides in tumour cells, 22.7/107 in non-tumour cells and 13.1/107 in blood leucocytes. There was a significantly higher level of N7-alkylguanines in the larynx tissues in males than in females. The effect of tobacco smoking on DNA adduct levels was shown by an increase in the average levels of N7-alkylguanines in the subject groups classified according to their smoking habits. A moderate age-related increase in levels of N7-alkylguanine was demonstrated in larynx tumour tissue. The levels of N7-alkylguanine adducts in larynx cells were compared with that of aromatic DNA adducts. Pearson correlation coefficients (0.28 for tumour tissue and 0.30 for non-tumour tissue) indicate independent formation and removal of N7-alkylguanine and aromatic DNA adducts resulting from tobacco smoke exposure.
ISSN:0143-3334
1460-2180
DOI:10.1093/carcin/17.3.501