Heteronuclear correlation experiments for the determination of one-bond coupling constants

Spin-state selective experiments, HSQC-alpha/beta and CT-HMQC-alpha/beta, are proposed for the simple and rapid measurement of scalar one-bond coupling constants in two-dimensional, 1H-detected 15N-1H or 13C-1H correlation experiments based on HSQC and HMQC schemes. Pairs of subspectra are obtained,...

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Bibliographic Details
Published in:Journal of biomolecular NMR 1998-05, Vol.11 (4), p.445-450
Main Authors: Andersson, P, Nordstrand, K, Sunnerhagen, M, Liepinsh, E, Turovskis, I, Otting, G
Format: Article
Language:English
Subjects:
Bacterial Proteins - chemistry
Data acquisition
Escherichia coli
Escherichia coli Proteins
Experiments
Medicin och hälsovetenskap
Nuclear Magnetic Resonance, Biomolecular - instrumentation
Nuclear Magnetic Resonance, Biomolecular - methods
Protein Structure, Secondary
Repressor Proteins - chemistry
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Summary:Spin-state selective experiments, HSQC-alpha/beta and CT-HMQC-alpha/beta, are proposed for the simple and rapid measurement of scalar one-bond coupling constants in two-dimensional, 1H-detected 15N-1H or 13C-1H correlation experiments based on HSQC and HMQC schemes. Pairs of subspectra are obtained, containing either the high-field or the low-field component of the doublet representing the one-bond coupling constant. The subspectral editing procedure retains the full sensitivity of HSQC and HMQC spectra recorded without heteronuclear decoupling during data acquisition, with a spectral resolution similar to that of decoupled spectra.
ISSN:0925-2738
1573-5001
DOI:10.1023/A:1008206212145