Characterization of canine anti-mouse antibodies highlights that multiple strategies are needed to combat immunoassay interference

Immunoassays are widely used for detection and quantification of analytes in biological samples, but are vulnerable to analytical errors caused by interfering sample substances. Of particular interest are endogenous anti-animal antibodies that may bind to the immunoassay antibodies and cause erroneo...

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Bibliographic Details
Published in:Scientific reports 2019-10, Vol.9 (1), p.14521-14521, Article 14521
Main Authors: Bergman, Daniel, Larsson, Anders, Hansson-Hamlin, Helene, Åhlén, Emma, Holst, Bodil Ström
Format: Article
Language:English
Subjects:
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Animals
Antibodies, Monoclonal - chemistry
Antibody Specificity
Biological samples
Clinical Science
Dogs
Enzyme-Linked Immunosorbent Assay - veterinary
Female
Humanities and Social Sciences
Immunization
Immunoassay
Immunoassays
Immunoglobulin A
Immunoglobulin A - chemistry
Immunoglobulin G
Immunoglobulin G - chemistry
Immunoglobulin M
Immunoglobulin M - chemistry
Immunoglobulins
Immunologi
Immunologic Tests
Immunology
Klinisk vetenskap
Male
Mice
multidisciplinary
Science
Science (multidisciplinary)
Species Specificity
Veterinary medicine
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Summary:Immunoassays are widely used for detection and quantification of analytes in biological samples, but are vulnerable to analytical errors caused by interfering sample substances. Of particular interest are endogenous anti-animal antibodies that may bind to the immunoassay antibodies and cause erroneous test results. This phenomenon is a hazard to patient safety in both human and veterinary medicine. Here, we demonstrate that anti-mouse antibodies in dogs bind selectively to different regions of the murine IgG molecule, cross-react with IgG from different species, and consist of all major antibody classes present in canine serum (IgA, IgG and IgM). The antibody characteristics varied among individuals and their prevalence differed between two dog breeds. The selective binding to different IgG regions suggests that the antibodies might not originate from immunization through exposure to mice or other species. These findings show that canine anti-mouse antibodies are highly heterogeneous in nature and therefore require a combination of strategies to be counteracted.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-51228-3