Seeking the true time: Exploring otolith chemistry as an age‐determination tool

• Published in: Journal of fish biology 2020-08, Vol.97 (2), p.552-565
• Main Authors: Heimbrand, Yvette, Limburg, Karin E., Hüssy, Karin, Casini, Michele, Sjöberg, Rajlie, Palmén Bratt, Anne‐Marie, Levinsky, Svend‐Erik, Karpushevskaia, Anastasia, Radtke, Krzysztof, Öhlund, Jill
• Format: Article
• Language: English
• Subjects: Age; Age determination; age estimation; Ageing; Aging; Animals; Annuli; Baltic Sea; Baltic States; Chronology; Clocks; Ecology; Ecosystem; Ekologi; Fish; Fish populations; Fisheries; Fisheries management; Fishery management; Gadus morhua; Gadus morhua - physiology; Growth rate; Growth rings; LA‐ICP‐MS; Marine fishes; Measuring instruments; Metabolism; Microchemistry; Migrations; North Sea; otolith chemistry; Otolithic Membrane - chemistry; Otoliths; Ratios; seasonal patterns; Seasonal variation; Seasonal variations; Seasonality; Stock assessment
• ISSN: 0022-1112; 1095-8649; 1095-8649
• DOI: 10.1111/jfb.14422

Fish otoliths' chronometric properties make them useful for age and growth rate estimation in fisheries management. For the Eastern Baltic Sea cod stock (Gadus morhua), unclear seasonal growth zones in otoliths have resulted in unreliable age and growth information. Here, a new age estimation method based on seasonal patterns in trace elemental otolith incorporation was tested for the first time and compared with the traditional method of visually counting growth zones, using otoliths from the Baltic and North seas. Various trace elemental ratios, linked to fish metabolic activity (higher in summer) or external environment (migration to colder, deeper habitats with higher salinity in winter), were tested for age estimation based on assessing their seasonal variations in concentration. Mg:Ca and P:Ca, both proxies for growth and metabolic activity, showed greatest seasonality and therefore have the best potential to be used as chemical clocks. Otolith image readability was significantly lower in the Baltic than in the North Sea. The chemical (novel) method had an overall greater precision and percentage agreement among readers (11.2%, 74.0%) than the visual (traditional) method (23.1%, 51.0%). Visual readers generally selected more highly contrasting zones as annuli whereas the chemical readers identified brighter regions within the first two annuli and darker zones thereafter. Visual estimates produced significantly higher, more variable ages than did the chemical ones. Based on the analyses in our study, we suggest that otolith microchemistry is a promising alternative ageing method for fish populations difficult to age, such as the Eastern Baltic cod.