1H, 13C and 15N backbone resonance assignment of Cel45A from Phanerochaete chrysosporium

A glycoside hydrolase family 45 (GH45) enzyme from the white-rot basidiomycete fungus Phanerochaete chrysosporium ( Pc Cel45A) was expressed in Pichia pastoris with 13 C and 15 N labelling. A nearly complete assignment of 1 H, 13 C and 15 N backbone resonances was obtained, as well as the secondary...

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Bibliographic Details
Published in:Biomolecular NMR assignments 2024, Vol.18 (2), p.153-157
Main Authors: Okmane, Laura, Sandgren, Mats, Ståhlberg, Jerry, Nestor, Gustav
Format: Article
Language:English
Subjects:
Biochemistry
Biochemistry and Molecular Biology
Biokemi och molekylärbiologi
Biological and Medical Physics
Biophysics
Glycoside hydrolase
Glycosides
Phanerochaete chrysosporium
Physics
Physics and Astronomy
Polymer Sciences
Protein structure
Secondary structure
Structural Biology
Strukturbiologi
White rot
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Summary:A glycoside hydrolase family 45 (GH45) enzyme from the white-rot basidiomycete fungus Phanerochaete chrysosporium ( Pc Cel45A) was expressed in Pichia pastoris with 13 C and 15 N labelling. A nearly complete assignment of 1 H, 13 C and 15 N backbone resonances was obtained, as well as the secondary structure prediction based on the assigned chemical shifts using the TALOS-N software. The predicted secondary structure was almost identical to previously published crystal structures of the same enzyme, except for differences in the termini of the sequence. This is the first NMR study using an isotopically labelled GH45 enzyme.
ISSN:1874-2718
1874-270X
1874-270X
DOI:10.1007/s12104-024-10182-6