Species-specific primers for predation studies of the pollen beetle, Meligethes aeneus (Coleoptera, Nitidulidae)

Species-specific primers were developed for the pollen beetle (a pest in oilseed rape) for studies of predation by natural insect predators. Two forward and three reverse primers were designed within the mitochondrial COI gene and used in combination to amplify fragments in the size range of 163-290...

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Bibliographic Details
Published in:Molecular ecology resources 2009-07, Vol.9 (4), p.1132-1134
Main Authors: CASSEL-LUNDHAGEN, ANNA, ÖBERG, SANDRA, HÖGFELDT, CAROL, EKBOM, BARBARA
Format: Article
Language:English
Subjects:
Agricultural Science
Araneae
biological control
Brassica napus var. napus
Coleoptera
Environmental Sciences related to Agriculture and Land-use
Jordbruksvetenskap
Meligethes aeneus
Miljö- och naturvårdsvetenskap
Nitidulidae
oilseed rape
Pardosa
predation
species-specific primers
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Summary:Species-specific primers were developed for the pollen beetle (a pest in oilseed rape) for studies of predation by natural insect predators. Two forward and three reverse primers were designed within the mitochondrial COI gene and used in combination to amplify fragments in the size range of 163-290 bp. Remains of pollen beetle DNA were consistently detected in Pardosa spiders up to 24 h after ingestion but dropped drastically at 48 h. These primers will facilitate studies on biological control of this oilseed rape pest. Detection time was not correlated with fragment length as might be expected as the DNA gradually degrades into progressively shorter fragments over time.
ISSN:1755-0998
1755-098X
1755-0998
DOI:10.1111/j.1755-0998.2009.02540.x