Diurnal oscillation of SBE expression in sorghum endosperm

• Published in: Journal of plant physiology 2009-01, Vol.166 (4), p.428-434
• Main Authors: Mutisya, Joel, Sun, Chuanxin, Rosenquist, Sara, Baguma, Yona, Jansson, Christer
• Format: Article
• Language: English
• Subjects: 1,4-alpha-Glucan Branching Enzyme - chemistry; 1,4-alpha-Glucan Branching Enzyme - genetics; 54; 58; Agricultural Science; Amino Acid Sequence; amino acid sequences; BARLEY; Biological and medical sciences; circadian rhythm; Circadian Rhythm - genetics; complementary DNA; DNA, Complementary - isolation & purification; DNA, Plant - isolation & purification; ENDOSPERM; enzyme activity; ENZYMES; Food Science; Fundamental and applied biological sciences. Psychology; Gene Dosage; Gene Expression Profiling; gene expression regulation; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; GENES; Genome, Plant - genetics; glucosyltransferases; grain sorghum; hexosyltransferases; Hordeum vulgare; Jordbruksvetenskap; Livsmedelsvetenskap; MAIZE; molecular cloning; Molecular Sequence Data; Oscillation; OSCILLATIONS; plant development; Plant physiology and development; PROBES; protein content; SBE; Seeds - enzymology; Seeds - genetics; Sequence Alignment; sequence analysis; Sequence Analysis, Protein; SORGHUM; Sorghum - enzymology; Sorghum - genetics; Sorghum bicolor; STARCH; starch branching enzyme; Zea mays
• ISSN: 0176-1617; 1618-1328; 1618-1328
• DOI: 10.1016/j.jplph.2008.06.017

Spatial and temporal expression patterns of the sorghum SBEI, SBEIIA and SBEIIB genes, encoding, respectively, starch branching enzyme (SBE) I, IIA and IIB, in the developing endosperm of sorghum ( Sorghum bicolor) were studied. Full-length genomic and cDNA clones for sorghum were cloned, and the SBEIIA cDNA was used together with gene-specific probes for sorghum SBEIIB and SBEI. In contrast to sorghum SBEIIB, which was expressed primarily in endosperm and embryo, SBEIIA was also expressed in vegetative tissues. All three genes shared a similar temporal expression profile during endosperm development, with a maximum activity at 15–24 d after pollination. This differed from barley and maize, in which SBEI gene activity showed a significantly later onset compared to that of SBEIIA and SBEIIB. Expression of the three SBE genes in the sorghum endosperm exhibited a diurnal rhythm during a 24-h cycle.