Identification of the Sex Pheromone of the Spruce Seed Moth, Cydia strobilella L

• Published in: Journal of chemical ecology 2010-03, Vol.36 (3), p.305-313
• Main Authors: Wang, Hong-Lei, Svensson, Glenn P, Rosenberg, Olle, Bengtsson, Marie, Jirle, Erling V, Löfstedt, Christer
• Format: Article
• Language: English
• Subjects: (8E,10E)-dodecadienyl acetate; (8E,10Z)-dodecadienyl acetate; Agriculture; Animal and plant ecology; Animal, plant and microbial ecology; Animals; antennae; Attractants; Bioassays; Biochemistry; Biologi; Biological and medical sciences; Biological Microscopy; Biological Sciences; Biomedical and Life Sciences; Butterflies & moths; chemical composition; Chromatography, Gas; Cydia strobilella; Ecology; electroantennography; Electrophysiology; Entomology; Environmental Sciences related to Agriculture and Land-use; Fatty Acids, Monounsaturated - chemistry; Fatty Acids, Monounsaturated - isolation & purification; Fatty Acids, Monounsaturated - pharmacology; Female; Fundamental and applied biological sciences. Psychology; Gas chromatography; Gas Chromatography-Mass Spectrometry; General aspects; Insect Control; Isomerism; Life Sciences; Mass spectrometry; Miljö- och naturvårdsvetenskap; Moths - physiology; Natural Sciences; Naturvetenskap; olfactometry; orchards; Pest control; pheromone blends; pheromone traps; Picea; sex attractants; Sex Attractants - chemistry; sex pheromones; spruce seed orchards; trapping; Trees; Zoologi; Zoology
• ISSN: 0098-0331; 1573-1561; 1573-1561
• DOI: 10.1007/s10886-010-9754-x

The spruce seed moth, Cydia strobilella L., is a serious pest on cones of spruce (Picea spp.) in the Holarctic region. Previous studies from different parts of its area of distribution have reported conflicting results on the composition of its sex pheromone. By gas chromatography with electroantennographic detection, coupled gas chromatography-mass spectrometry, a Y-tube olfactometer bioassay, and field trials, the sex pheromone of Swedish populations of the species was identified as (8E,10E)-dodecadienyl acetate and (8E,10Z)-dodecadienyl acetate. About 0.5 pg of each pheromone component was extracted per female. The most attractive blend of EE- and EZ-isomers was about 6:4, respectively, and 0.3 µg of the blend per rubber septum was the most attractive dosage for field trapping. Monounsaturated components previously reported as sex pheromone components/attractants for C. strobilella, (E)-8-dodecenyl acetate in Canadian populations and (Z)-8-dodecenol in Polish and Dutch populations, did not attract any C. strobilella in this study. Large numbers of C. jungiella Clerck were trapped by using (8E,10Z)-dodecadienyl acetate alone, whereas (Z)-8-dodecenol attracted Pammene splendidulana Guenée and P. rhediella Clerck.