Phylogenetic analysis of bovine respiratory syncytial viruses from recent outbreaks in feedlot and dairy cattle herds

• Published in: Archives of virology 2012-04, Vol.157 (4), p.601-607
• Main Authors: Bidokhti, Mehdi R. M., Tråvén, Madeleine, Ohlson, Anna, Zarnegar, Behdad, Baule, Claudia, Belák, Sándor, Alenius, Stefan, Liu, Lihong
• Format: Article
• Language: English
• Subjects: Animal and Dairy Science; Animals; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Bovine respiratory syncytial virus; Cattle; Cattle Diseases - epidemiology; Cattle Diseases - virology; Cluster Analysis; Dairy cattle; Disease Outbreaks; Epidemics; Epidemiology; Fundamental and applied biological sciences. Psychology; Genetic Variation; Genomes; Genotype; Glycoproteins; Husdjursvetenskap; Infections; Infectious Diseases; Medical Microbiology; Microbiology; Miscellaneous; Molecular Epidemiology; Molecular Sequence Data; Original Article; Phylogenetics; Phylogeny; Polymerase Chain Reaction; Proteins; Respiratory diseases; Respiratory syncytial virus; Respiratory Syncytial Virus Infections - epidemiology; Respiratory Syncytial Virus Infections - veterinary; Respiratory Syncytial Virus Infections - virology; Respiratory Syncytial Virus, Bovine - classification; Respiratory Syncytial Virus, Bovine - genetics; Respiratory Syncytial Virus, Bovine - isolation & purification; RNA, Viral - genetics; Sequence Analysis, DNA; Sweden - epidemiology; Veterinary Science; Veterinärmedicin; Viral Envelope Proteins - genetics; Virology; Viruses
• ISSN: 0304-8608; 1432-8798; 1432-8798
• DOI: 10.1007/s00705-011-1209-3

Bovine respiratory syncytial virus (BRSV) is one of the major causes of bovine respiratory disease worldwide. In order to study the molecular epidemiology of the virus, samples from 30 BRSV outbreaks in cattle herds located in different parts of Sweden were collected from 2007 to 2011. The samples were analyzed by PCR, and the glycoprotein (G) gene was sequenced. BRSV was detected in outbreaks of respiratory disease in both dairy and feedlot herds most often during the winter period but also during the summer months (May to August). This indicates that circulation of the virus between herds occurs throughout the year. Comparative sequence analysis revealed a high degree (more than 94.5%) of sequence identity among the collected strains. Phylogenetic analysis showed that 29 out of the 30 strains formed a unique clade. Identical sequences found in herds sampled within a few months’ time suggested that these herds were part of a common transmission chain. One strain from a single outbreak in a herd in southern Sweden clustered with Danish strains and showed a distant relationship to the rest of the Swedish strains. Further studies are highly warranted to clarify the inter-herd transmission routes of BRSV. Such knowledge is essential for the control of the spread of this virus between herds, regions and even countries.