Subtyping of Listeria monocytogenes isolates recovered from retail ready-to-eat foods, processing plants and listeriosis patients in Sweden 2010

• Published in: International journal of food microbiology 2013-08, Vol.166 (1), p.186-192
• Main Authors: Lambertz, S. Thisted, Ivarsson, S., Lopez-Valladares, G., Sidstedt, M., Lindqvist, R.
• Format: Article
• Language: English
• Subjects: Aged; Animals; Biological and medical sciences; Culinary Arts and Meal Science; Electrophoresis, Gel, Pulsed-Field; Environmental Microbiology; Female; Food Handling; Food industries; Food Microbiology; Food Science; Fundamental and applied biological sciences. Psychology; Genotype; Humans; Listeria monocytogenes; Listeria monocytogenes - classification; Listeria monocytogenes - genetics; Listeria monocytogenes - isolation & purification; Listeriosis - epidemiology; Listeriosis - microbiology; Livsmedelsvetenskap; Male; Microbiology; Mikrobiologi; Måltidskunskap; PFGE; Polymerase Chain Reaction; Serotyping; Sweden - epidemiology; Typing
• ISSN: 0168-1605; 1879-3460; 1879-3460
• DOI: 10.1016/j.ijfoodmicro.2013.06.008

Identification and prioritisation of food safety interventions requires an understanding of the relationship between food, pathogens and cases. Such understanding can be gained through different approaches, e.g. microbial subtyping to attribute cases of foodborne disease to food vehicles or other sources of illness. In this study, Listeria monocytogenes isolates (n=166) from (i) three categories of ready-to-eat (RTE) foods, (ii) food processing plant environments, and (iii) human listeriosis cases, all sampled during 2010 in Sweden, were subtyped. In addition, 121 isolates from human listeriosis cases, collected 2005–2009, were subtyped. Subtyping consisted of both serotyping (conventional method and PCR) and genotyping using pulsed-field gel electrophoresis (PFGE). Serotype 1/2a dominated in all three groups of isolates (range 73–96%). Eighteen percent of the human isolates (2010) belonged to serotype 4b, but only 1.4% of the food isolates. The food isolates differentiated into 19 pulsotypes (ID=0.843), the human isolates collected 2010 into 31 pulsotypes (ID=0.950) and the processing plant isolates into 22 pulsotypes (ID=0.991). Six of the pulsotypes were shared between the food and human isolates. These pulsotypes comprised 42% of the human isolates and 59% of the food isolates. For some processing plants, there was suggested persistence of one or more specific L. monocytogenes strains, as indicated by repetitive isolation of the same pulsotype from food. This study indicated the presence of L. monocytogenes in the processing plant environment as a likely source of contamination of gravad and cold-smoked fish, and this food category as an important source of human exposure to the pathogen. •42% of the pulsotypes displayed by human isolates were also found in food isolates.•93% of food isolates were found on at least one occasion among human isolates.•Persistence of one or more specific L. monocytogenes strains was indicated in plants.