Genotypic variation of nodules’ enzymatic activities in symbiotic nitrogen fixation among common bean (Phaseolus vulgaris L.) genotypes grown under salinity constraint

• Published in: Symbiosis (Philadelphia, Pa.) Pa.), 2013, Vol.60 (3), p.115-122
• Main Authors: Faghire, Mustapha, Mohamed, Farissi, Taoufiq, Khadijattou, Fghire, Rachid, Bargaz, Adnane, Mandri, Btissam, Oufdou, Khalid, Laury, Amenc, Drevon, Jean-Jacques, Ghoulam, Cherki
• Format: Article
• Language: English
• Subjects: acid phosphatase; Animal, plant and microbial ecology; beans; Biochemistry and Molecular Biology; Biokemi och molekylärbiologi; Biological and medical sciences; biomass; Biomedical and Life Sciences; cultivars; Developmental Biology; Ecology; enzyme activity; Evolutionary Biology; Fundamental and applied biological sciences. Psychology; genotype; greenhouses; inbred lines; Life Sciences; Microbiology; nitrogen fixation; nodulation; Phaseolus vulgaris; Plant Sciences; salinity; salt stress; salt tolerance; shoots; sodium chloride; symbiosis; trehalose
• ISSN: 0334-5114; 1878-7665; 1878-7665
• DOI: 10.1007/s13199-013-0247-x

The effect of salt stress, under glasshouse conditions, was studied on plant biomass, nodulation, and activities of acid phosphatases (APase, EC 3.1.3.2) and trehalose 6-phosphate phosphatase (TPP, EC 3.1.3.12) in the symbiosis common bean (Phaseolus vulgaris L.)-rhizobia nodules. Four common bean recombinant inbred lines (147, 115, 104 and 83) were separately inoculated, with CIAT 899 or RhM11 strains and grown in hydroaeroponic culture. Two NaCl levels (0 and 25 mM NaCl plant⁻¹ week⁻¹ corresponding, respectively, to the control and the salt treatment) were applied and the culture was assessed during 42 days after their transplantation. The results showed that the nodulation of these lines was not affected by salinity except for the line 83 inoculated with CIAT 899, whose nodule dry weight decreased by 48.24 % compared with the corresponding controls. For the other symbiotic combinations, shoot and root biomasses were not significantly affected by salt constraint. Salinity stress generally reduced acid phosphatise and trehalose phosphate phosphatase activities in nodules that were less affected in plants inoculated with RhM11. Based on our data, it appears that nodule phosphatase activity may be involved in salinity tolerance in common beans and the levels of salt tolerance depend principally on specific combination of the rhizobial strain and the host cultivar.