The genes of all seven CYP3A isoenzymes identified in the equine genome are expressed in the airways of horses

• Published in: Journal of veterinary pharmacology and therapeutics 2013-08, Vol.36 (4), p.370-375
• Main Authors: Tydén, E., Löfgren, M., Hakhverdyan, M., Tjälve, H., Larsson, P.
• Format: Article
• Language: English
• Subjects: Animals; Cytochrome P-450 CYP3A - classification; Cytochrome P-450 CYP3A - genetics; Cytochrome P-450 CYP3A - metabolism; Female; Gene Expression Regulation, Enzymologic - physiology; Genome; Horses - genetics; Horses - metabolism; Isoenzymes; Male; Pathobiology; Patobiologi; Respiratory System - enzymology; Respiratory System - metabolism
• ISSN: 0140-7783; 1365-2885; 1365-2885
• DOI: 10.1111/jvp.12012

In the present study, we examined the gene expression of cytochrome P450 3A (CYP3A) isoenzymes in the tracheal and bronchial mucosa and in the lung of equines using TaqMan probes. The results show that all seven CYP3A isoforms identified in the equine genome, that is, CYP3A89, CYP3A93, CYP3A94, CYP3A95, CYP3A96, CYP3A97 and CYP3A129, are expressed in the airways of the investigated horses. Though in previous studies, CYP3A129 was found to be absent in equine intestinal mucosa and liver, this CYP3A isoform is expressed in the airways of horses. The gene expression of the CYP3A isoenzymes varied considerably between the individual horses studied. However, in most of the horses CYP3A89, CYP3A93, CYP3A96, CYP3A97 and CYP3A129 were expressed to a high extent, while CYP3A94 and CYP3A95 were expressed to a low extent in the different parts of the airways. The CYP3A isoenzymes present in the airways may play a role in the metabolic degradation of inhaled xenobiotics. In some instances, the metabolism may, however, result in bioactivation of the xenobiotics and subsequent tissue injury.