Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

Flow cytometry is now a recognized methodology within animal spermatology, and has moved from being a research tool to become routine in the assessment of animal semen destined to breeding. The availability of 'bench-top' flow cytometers and of newer and versatile markers for cell structur...

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Bibliographic Details
Published in:Asian journal of andrology 2011-05, Vol.13 (3), p.406-419
Main Authors: Hossain, Md Sharoare, Johannisson, Anders, Wallgren, Margareta, Nagy, Szabolcs, Siqueira, Amanda Pimenta, Rodriguez-Martinez, Heriberto
Format: Article
Language:English
Subjects:
Acrosome - physiology
Animal and Dairy Science
Animals
Cell Survival
Chromatin - chemistry
Flow Cytometry - methods
Husdjursvetenskap
Lipid Peroxidation
Male
MEDICIN
MEDICINE
Mitochondria - physiology
Oxidative Stress
Review
Semen Analysis - methods
Semen Preservation - methods
Sex Preselection - methods
Sperm Capacitation
Spermatozoa - cytology
Spermatozoa - physiology
Veterinary Science
Veterinärmedicin
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Summary:Flow cytometry is now a recognized methodology within animal spermatology, and has moved from being a research tool to become routine in the assessment of animal semen destined to breeding. The availability of 'bench-top' flow cytometers and of newer and versatile markers for cell structure and function had allowed the instrumentation to measure more sperm parameters, from viability to reactiveness when exposed to exogenous stimuli, and to increase our capabilities to sort spermatozoa for potential fertilizing capacity, or chromosomal sex. The present review summarizes the state of the art regarding flow cytometry applied to animal andrology, albeit keeping an open comparative intent. It critically evaluates the present and future capabilities of flow cytometry for the diagnostics of potential fertility and for the development of current reproductive technologies such as sperm freezing, sperm selection and sperm sorting. The flow cytometry methods will probably further revolutionize our understanding of the sperm physiology and their functionality, and will undoubtedly extend its application in isolating many uncharacterized features of spermatozoa. However, continuous follow-up of the methods is a necessity owing to technical developments and the complexity of mapping spermatozoa.
ISSN:1008-682X
1745-7262
1745-7262
DOI:10.1038/aja.2011.15