An Intrinsic MicroRNA Timer Regulates Progressive Decline in Shoot Regenerative Capacity in Plants

Plant cells are totipotent and competent to regenerate from differentiated organs. It has been shown that two phytohormones, auxin and cytokinin, play critical roles within this process. As in animals, the regenerative capacity declines with age in plants, but the molecular basis for this phenomenon...

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Bibliographic Details
Published in:The Plant cell 2015-02, Vol.27 (2), p.349-360
Main Authors: Zhang, Tian-Qi, Lian, Heng, Tang, Hongbo, Dolezal, Karel, Zhou, Chuan-Miao, Yu, Sha, Chen, Juan-Hua, Chen, Qi, Liu, Hongtao, Ljung, Karin, Wang, Jia-Wei
Format: Article
Language:English
Subjects:
Arabidopsis
Arabidopsis - genetics
Arabidopsis - physiology
Auxins
Biochemistry and Molecular Biology
Biokemi och molekylärbiologi
Botanik
Botany
Cytokinins
Cytokinins - pharmacology
Gene expression regulation
Genes, Plant
Hypocotyls
Leaves
MicroRNA
MicroRNAs - genetics
MicroRNAs - metabolism
Nicotiana - genetics
Nicotiana - physiology
Plant cells
Plant Proteins - metabolism
Plant Shoots - genetics
Plant Shoots - physiology
Plants
Regeneration - genetics
Transactivation
Yeasts
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Summary:Plant cells are totipotent and competent to regenerate from differentiated organs. It has been shown that two phytohormones, auxin and cytokinin, play critical roles within this process. As in animals, the regenerative capacity declines with age in plants, but the molecular basis for this phenomenon remains elusive. Here, we demonstrate that an age-regulated microRNA, miR156, regulates shoot regenerative capacity. As a plant ages, the gradual increase in miR156-targeted SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors leads to the progressive decline in shoot regenerative capacity. In old plants, SPL reduces shoot regenerative capacity by attenuating the cytokinin response through binding with the B-type ARABIDOPSIS RESPONSE REGULATORs, which encode the transcriptional activators in the cytokinin signaling pathway. Consistently, the increased amount of exogenous cytokinin complements the reduced shoot regenerative capacity in old plants. Therefore, the recruitment of age cues in response to cytokinin contributes to shoot regenerative competence.
ISSN:1040-4651
1532-298X
1532-298X
DOI:10.1105/tpc.114.135186