Short communication: Lipolytic activity on milk fat by Staphylococcus aureus and Streptococcus agalactiae strains commonly isolated in Swedish dairy herds

• Published in: Journal of dairy science 2015-12, Vol.98 (12), p.8560-8564
• Main Authors: Vidanarachchi, Janak K., Li, Shengjie, Lundh, Åse Sternesjö, Johansson, Monika
• Format: Article
• Language: English
• Subjects: Agricultural Science; Animal and Dairy Science; Animals; bovine milk; Cattle; Fatty Acids, Nonesterified - metabolism; Female; Food Science; free fatty acids; Husdjursvetenskap; Jordbruksvetenskap; Lipase - metabolism; Lipids - analysis; Lipolysis; Livsmedelsvetenskap; mastitis bacteria; Mastitis, Bovine - microbiology; Milk - chemistry; Milk - microbiology; Staphylococcal Infections - veterinary; Staphylococcus aureus - enzymology; Staphylococcus aureus - isolation & purification; Streptococcus agalactiae - enzymology; Streptococcus agalactiae - isolation & purification; Sweden; thin-layer chromatography
• ISSN: 0022-0302; 1525-3198; 1525-3198
• DOI: 10.3168/jds.2015-9559

The objective of this study was to determine the lipolytic activity on milk fat of 2 bovine mastitis pathogens, that is, Staphylococcus aureus and Streptococcus agalactiae. The lipolytic activity was determined by 2 different techniques, that is, thin-layer chromatography and an extraction-titration method, in an experimental model using the most commonly occurring field strains of the 2 mastitic bacteria isolated from Swedish dairy farms. The microorganisms were inoculated into bacteria-free control milk and incubated at 37°C to reflect physiological temperatures in the mammary gland. Levels of free fatty acids (FFA) were analyzed at time of inoculation (t=0) and after 2 and 6h of incubation, showing significant increase in FFA levels. After 2h the FFA content had increased by approximately 40% in milk samples inoculated with Staph. aureus and Strep. agalactiae, and at 6h the pathogens had increased FFA levels by 47% compared with the bacteria-free control milk. Changes in lipid composition compared with the bacteria-free control were investigated at 2 and 6h of incubation. Diacylglycerols, triacylglycerols, and phospholipids increased significantly after 6h incubation with the mastitis bacteria, whereas cholesterol and sterol esters decreased. Our results suggest that during mammary infections with Staph. aureus and Strep. agalactiae, the action of lipases originating from the mastitis pathogens will contribute significantly to milk fat lipolysis and thus to raw milk deterioration.