Present genetic structure is congruent with the common origin of distant Scots pine populations in its Romanian distribution

• Published in: Forest ecology and management 2016-02, Vol.361, p.131-143
• Main Authors: Bernhardsson, C., Floran, V., Ganea, S.L., García-Gil, M.R.
• Format: Article
• Language: English
• Subjects: Chloroplast SSR; Ecology; Evolutionary; Forest Science; Genetic diversity; Genetics; Genomes; Hungary; Loci; Nad1/nad7 mitotype; Nuclear SSR; Origins; Pine; Pinus cembra; Pinus sylvestris; Populations; Romania; Skogsvetenskap; Spatial genetic structure
• ISSN: 0378-1127; 1872-7042; 1872-7042
• DOI: 10.1016/j.foreco.2015.10.047

•Romanian Scots pine populations have maintained high levels of genetic diversity.•The level of inbreeding reflects a history of contraction.•Nuclear, cp and mt DNA is congruent with their model of inheritance, ploidy and dispersion.•Genetic structure is congruent with a history of glacial expansion and posterior contraction. Owing to the fact that mitochondrial, chloroplast and nuclear genomes are differently affected by evolutionary forces, the comparative study of all three genomes in combination with paleobotanical evidences allows for a more accurate interpretation of present genetic make-up. In this study, we analyzed the nad1–nad7 mitotype, 14 cpSSR loci and 10 nSSR loci in 13 Romanian Scots pine (Pinus sylvestris L.) populations from the Romanian Carpathians and two Hungarian populations. Previous analysis of the macrofossil records supports the presence of Scots pine and Swiss stone pine (Pinus cembra L.) during the glacial period in Hungary and Romania. For a better image into the population structure in the studied area we also included in the data analysis two outgroup populations (north and south of Sweden) of known post-glacial origin, which resulted in a more defined genetic structure. Our study reveals that relatively high genetic diversity is preserved at all the Romanian and Hungarian populations, with no distinction among naturally regenerated, managed autochthonous and introduced populations. Furthermore, genetic differentiation analysis fails to distinguish the two introduced populations from the Romanian ones. AMOVA clustering is congruent with the paleobotanical-based evidences of contraction of Scots pine range since the Holocene to its present fragmented distribution.