Cytokinin and Auxin Display Distinct but Interconnected Distribution and Signaling Profiles to Stimulate Cambial Activity

• Published in: Current biology 2016-08, Vol.26 (15), p.1990-1997
• Main Authors: Immanen, Juha, Nieminen, Kaisa, Smolander, Olli-Pekka, Kojima, Mikiko, Alonso Serra, Juan, Koskinen, Patrik, Zhang, Jing, Elo, Annakaisa, Mähönen, Ari Pekka, Street, Nathaniel, Bhalerao, Rishikesh P., Paulin, Lars, Auvinen, Petri, Sakakibara, Hitoshi, Helariutta, Ykä
• Format: Article
• Language: English
• Subjects: Cambium - growth & development; Cell Biology; Cellbiologi; Cytokinins - metabolism; Genome, Plant; Indoleacetic Acids - metabolism; Plant Growth Regulators - metabolism; Plants, Genetically Modified - genetics; Plants, Genetically Modified - growth & development; Plants, Genetically Modified - physiology; Populus - genetics; Populus - growth & development; Populus - physiology; Signal Transduction; Transcriptome
• ISSN: 0960-9822; 1879-0445; 1879-0445
• DOI: 10.1016/j.cub.2016.05.053

Despite the crucial roles of phytohormones in plant development, comparison of the exact distribution profiles of different hormones within plant meristems has thus far remained scarce. Vascular cambium, a wide lateral meristem with an extensive developmental zonation, provides an optimal system for hormonal and genetic profiling. By taking advantage of this spatial resolution, we show here that two major phytohormones, cytokinin and auxin, display different yet partially overlapping distribution profiles across the cambium. In contrast to auxin, which has its highest concentration in the actively dividing cambial cells, cytokinins peak in the developing phloem tissue of a Populus trichocarpa stem. Gene expression patterns of cytokinin biosynthetic and signaling genes coincided with this hormonal gradient. To explore the functional significance of cytokinin signaling for cambial development, we engineered transgenic Populus tremula × tremuloides trees with an elevated cytokinin biosynthesis level. Confirming that cytokinins function as major regulators of cambial activity, these trees displayed stimulated cambial cell division activity resulting in dramatically increased (up to 80% in dry weight) production of the lignocellulosic trunk biomass. To connect the increased growth to hormonal status, we analyzed the hormone distribution and genome-wide gene expression profiles in unprecedentedly high resolution across the cambial zone. Interestingly, in addition to showing an elevated cambial cytokinin content and signaling level, the cambial auxin concentration and auxin-responsive gene expression were also increased in the transgenic trees. Our results indicate that cytokinin signaling specifies meristematic activity through a graded distribution that influences the amplitude of the cambial auxin gradient. •Gene expression was profiled globally across the cambium in high resolution•Auxin and cytokinin display distinct distribution profiles across the cambium•Increased cytokinin content and signaling level stimulate cambial cell divisions•Elevation of cytokinin content leads to an increased cambial auxin concentration A new report explores how two major phytohormones, cytokinin and auxin, contribute to the control of tree trunk growth. Immanen et al. show that by boosting cytokinin biosynthesis, they can both increase auxin level and stimulate lignocellulosic biomass production. Both hormones represent optimal targets for tree breeding and forest biotechnology