The Bacterial (Vibrio alginolyticus) Production of Tetrodotoxin in the Ribbon Worm Lineus longissimus-Just a False Positive?

We test previous claims that the bacteria Vibrio alginolyticus produces tetrodotoxin (TTX) when living in symbiosis with the nemertean Lineus longissimus by a setup with bacteria cultivation for TTX production. Toxicity experiments on the shore crab, Carcinus maenas, demonstrated the presence of a p...

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Bibliographic Details
Published in:Marine drugs 2016-03, Vol.14 (4), p.63-63
Main Authors: Strand, Malin, Hedström, Martin, Seth, Henrik, McEvoy, Eric G, Jacobsson, Erik, Göransson, Ulf, Andersson, Håkan S, Sundberg, Per
Format: Article
Language:English
Subjects:
1988
1992
Animals
Axonal conductance
bay
Biochemistry and Molecular Biology
Biokemi och molekylärbiologi
Brachyura - microbiology
Brachyura - parasitology
Carcinus maenas
cephalothrix-simula
Chromatography, Liquid - methods
Decapoda
Engineering and Technology
Environmental Biotechnology
fishes
gated sodium-channels
Helminths - microbiology
hiroshima prefecture
japan
LC-MS
Lineus
Lineus longissimus
llens me
Male
Marine
Medical Chemistry
Medicinsk kemi
Miljöbioteknik
Molecular Weight
Mucus
Nemertean
nemertiner
Oceanografi, hydrologi, vattenresurser
Oceanography, Hydrology, Water Resources
p867
Paralysis - chemically induced
Pharmacology & Pharmacy
platyhelminthes
polycladida
proceedings of the national academy of sciences of the united states of
puffer
Rats
Rats, Wistar
slemmaskar
substances
Symbiosis - physiology
Tandem Mass Spectrometry - methods
Teknik
Tetrodotoxin
Tetrodotoxin - toxicity
toxicity
toxicon
v26
Vibrio
Vibrio alginolyticus
Vibrio alginolyticus - metabolism
Voltage-Gated Sodium Channels - metabolism
yazawa k
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Summary:We test previous claims that the bacteria Vibrio alginolyticus produces tetrodotoxin (TTX) when living in symbiosis with the nemertean Lineus longissimus by a setup with bacteria cultivation for TTX production. Toxicity experiments on the shore crab, Carcinus maenas, demonstrated the presence of a paralytic toxin, but evidence from LC-MS and electrophysiological measurements of voltage-gated sodium channel-dependent nerve conductance in male Wistar rat tissue showed conclusively that this effect did not originate from TTX. However, a compound of similar molecular weight was found, albeit apparently non-toxic, and with different LC retention time and MS/MS fragmentation pattern than those of TTX. We conclude that C. maenas paralysis and death likely emanate from a compound
ISSN:1660-3397
1660-3397
DOI:10.3390/md14040063