Estrogen Down-Regulates mRNA Encoding the Exocytotic Protein SNAP-25 in the Rat Pituitary Gland

Exocytosis is dependent on specific proteins that are located at the secretory granule membrane, in the cytoplasm or at the plasma membrane. The mRNA expression of synaptosomal‐associated protein of 25 kDa (SNAP‐25) isoforms SNAP‐25a and SNAP‐25b, vesicle associated membrane protein (VAMP) 2, mammal...

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Published in:Journal of neuroendocrinology 1998-03, Vol.10 (3), p.157-163
Main Authors: Jacobsson, Gunilla, Razani, Haleh, Ogren, Sven-Ove, Meister, Björn
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - genetics
Animals
Drug Implants
Endosomal Sorting Complexes Required for Transport
Estradiol - pharmacology
Exocytosis - physiology
Female
In Situ Hybridization
Medicin och hälsovetenskap
Membrane Proteins - genetics
Munc18 Proteins
Nerve Tissue Proteins - genetics
Nerve Tissue Proteins - physiology
Ovariectomy
Phosphoproteins
Pituitary Gland - metabolism
R-SNARE Proteins
Rats
Rats, Sprague-Dawley
RNA, Messenger - metabolism
Synaptosomal-Associated Protein 25
Vesicular Transport Proteins
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Summary:Exocytosis is dependent on specific proteins that are located at the secretory granule membrane, in the cytoplasm or at the plasma membrane. The mRNA expression of synaptosomal‐associated protein of 25 kDa (SNAP‐25) isoforms SNAP‐25a and SNAP‐25b, vesicle associated membrane protein (VAMP) 2, mammalian homologue of unc‐18 (munc‐18) and Hrs‐2 was studied in the pituitary of ovariectomized rats after subcutaneous insertion of capsules containing estrogen or placebo using in situ hybridization. Estrogen treatment (0.25 mg estradiol) significantly decreased SNAP‐25a (32%; 10%) and SNAP‐25b (25%; 22%) mRNA levels in the anterior and intermediate lobes, respectively, whereas VAMP‐2, munc‐18 and Hrs‐2 mRNA levels remained unchanged. The results suggest that estrogen selectively regulates SNAP‐25 transcription in the pituitary gland, but leaves VAMP‐2, munc‐18 and Hrs‐2 mRNA levels unaffected.
ISSN:0953-8194
1365-2826
DOI:10.1046/j.1365-2826.1998.00178.x