Effect of nitric oxide on single skeletal muscle fibres from the mouse

Single skeletal muscle fibres from a mouse foot muscle were used to investigate the effects of nitric oxide on contractile function. We measured force production and myoplasmic free Ca 2+ concentration ([Ca 2+ ] i ) in single fibres exposed to the nitric oxide donors S -nitroso- N -acetylcysteine (S...

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Bibliographic Details
Published in:The Journal of physiology 1998-06, Vol.509 (2), p.577-586
Main Authors: Andrade, Francisco H., Reid, Michael B., Allen, David G., Westerblad, Håkan
Format: Article
Language:English
Subjects:
Acetylcysteine - analogs & derivatives
Acetylcysteine - pharmacology
Aminoquinolines - pharmacology
Animals
Calcium - metabolism
Enzyme Inhibitors - pharmacology
Guanylate Cyclase - antagonists & inhibitors
In Vitro Techniques
Kinetics
Male
Medicin och hälsovetenskap
Mice
Mice, Inbred Strains
Muscle Contraction - drug effects
Muscle Fibers, Skeletal - drug effects
Muscle Fibers, Skeletal - physiology
Muscle, Skeletal - drug effects
Muscle, Skeletal - physiology
Myofibrils - drug effects
Myofibrils - physiology
Nitric Oxide - pharmacology
Nitroprusside - pharmacology
Original
Sodium Nitrite - pharmacology
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Summary:Single skeletal muscle fibres from a mouse foot muscle were used to investigate the effects of nitric oxide on contractile function. We measured force production and myoplasmic free Ca 2+ concentration ([Ca 2+ ] i ) in single fibres exposed to the nitric oxide donors S -nitroso- N -acetylcysteine (SNAC) and nitroprusside. The nitric oxide donors reduced myofibrillar Ca 2+ sensitivity, whereas [Ca 2+ ] i transients were increased during submaximal tetani. Force was largely unchanged. SNAC did not change maximum shortening velocity, the rate of force redevelopment, or force production at saturating [Ca 2+ ] i . The guanylyl cyclase inhibitor LY83583 increased tetanic [Ca 2+ ] i but had no effect on Ca 2+ sensitivity. LY83583 did not prevent the decrease in myofibrillar Ca 2+ sensitivity in response to SNAC. The oxidizer sodium nitrite increased tetanic [Ca 2+ ] i and decreased myofibrillar Ca 2+ sensitivity. We conclude that under our experimental conditions nitric oxide impairs Ca 2+ activation of the actin filaments which results in decreased myofibrillar Ca 2+ sensitivity.
ISSN:0022-3751
1469-7793
DOI:10.1111/j.1469-7793.1998.577bn.x