5-lipoxygenase Expression in Langerhans Cells of Normal Human Epidermis

We studied expression of the 5-lipoxygenase (5-LO) pathway in normal human skin. In situ hybridization revealed a 5-LO mRNA-containing epidermal cell (EC) population that was predominantly located in the midportion of the spinous layer, in outer hair root sheaths, and in the epithelial compartment o...

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Published in:Proceedings of the National Academy of Sciences - PNAS 1998-01, Vol.95 (2), p.663-668
Main Authors: Spanbroek, Rainer, Stark, Hans-Jurgen, Janen-Timmen, Uwe, Kraft, Stefan, Hildner, Markus, Andl, Thomas, Bosch, Franz-Xaver, Fusenig, Norbert E., Bieber, Thomas, Rdmark, Olof, Samuelsson, Bengt, Andreas J. R. Habenicht
Format: Article
Language:English
Subjects:
Arachidonate 5-Lipoxygenase - biosynthesis
Biological Sciences
Cell lines
Cellular biology
Cellular immunity
Dendritic cells
Epidermal cells
Epidermis
Epidermis - enzymology
Humans
Immunology
In Situ Hybridization
Keratinocytes
Langerhans Cells - enzymology
Leukocytes
Mast cells
Proteins
Reverse transcriptase polymerase chain reaction
RNA, Messenger - analysis
Skin
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Summary:We studied expression of the 5-lipoxygenase (5-LO) pathway in normal human skin. In situ hybridization revealed a 5-LO mRNA-containing epidermal cell (EC) population that was predominantly located in the midportion of the spinous layer, in outer hair root sheaths, and in the epithelial compartment of sebaceous glands. Examination of skin specimens by immunohistochemistry and of primary ECs by flow cytometry mapped the 5-LO protein exclusively to Langerhans cells (LCs). The LC 5-LO protein was largely found in the nuclear matrix, in nuclear envelopes, and perinuclear regions as indicated by in situ confocal laser scan microscopy. Reverse transcription-PCR and immunoblot analyses of purified primary EC populations further indicated that LCs are major 5-LO expressing cells. Enriched primary LCs were also found to contain 5-LO activating protein (FLAP), leukotriene (LT) C4synthase, and LTA4hydrolase. By contrast, 5-LO, FLAP, and LTC4synthase were undetectable or largely reduced, but LTA4hydrolase transcripts and protein were identified in ECs depleted of LCs. These data show that naive LCs are major, and possibly the sole, 5-LO pathway expressing cells in the normal human epidermis.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.95.2.663