The effect of stereocilia bundle position on receptor potential characteristics of isolated outer hair cells in the guinea pig

The receptor potential as a function of pre-stimulus position of the stereocilia was studied with the whole cell patch-clamp technique in isolated outer hair cells (OHC) during direct mechanical stimulation of the stereocilia ( n=6, cells ranging from 70–87 μm length). The amplitude and frequency of...

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Bibliographic Details
Published in:Neuroscience letters 1997-09, Vol.234 (1), p.55-58
Main Authors: Shvarev, Yuri N, Canlon, Barbara
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Auditory system
Cilia - physiology
Cochlea
Guinea pig
Guinea Pigs
Hair Cells, Auditory, Outer - physiology
In Vitro Techniques
Mechanoelectrical transduction
Mechanoreceptors - physiology
Medicin och hälsovetenskap
Membrane Potentials - physiology
Outer hair cell
Patch-Clamp Techniques
Receptor potential
Stereocilia
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Summary:The receptor potential as a function of pre-stimulus position of the stereocilia was studied with the whole cell patch-clamp technique in isolated outer hair cells (OHC) during direct mechanical stimulation of the stereocilia ( n=6, cells ranging from 70–87 μm length). The amplitude and frequency of the stimulation was 1 μm and 100 Hz. Controlled pre-stimulus stereocilia displacements in the excitatory direction were followed by sustained membrane depolarization and a diminution of the DC component of the receptor potential. Spectral analysis has shown that recordings with most diminished DC components had a pronounced fundamental while the other harmonics were suppressed, indicating the linearization of the receptor potential. The observed non-lineararities depended on the resting position of the stereocilia and seemed to be determined by the operating range of the transduction process.
ISSN:0304-3940
1872-7972
DOI:10.1016/S0304-3940(97)00672-1