nrdD and nrdG genes are essential for strict anaerobic growth of Escherichia coli

In Escherichia coli ribonucleotide reduction is catalyzed by two separate enzymes during aerobic and anaerobic growth. The aerobic enzyme is coded by the nrdAB genes, the anaerobic enzyme by nrdDG. We now show that knock-out mutants of either nrdD or nrdG cannot grow during strict anaerobiosis, achi...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1996-12, Vol.229 (1), p.189-192
Main Authors: Garriga, X, Eliasson, R, Torrents, E, Jordan, A, Barbé, J, Gibert, I, Reichard, P
Format: Article
Language:English
Subjects:
Aerobiosis
Anaerobiosis
Bacterial Proteins - metabolism
Escherichia coli - genetics
Escherichia coli - growth & development
Escherichia coli Proteins
Gene Expression Regulation, Bacterial
Genes, Bacterial
Hydroxyurea - pharmacology
Medicin och hälsovetenskap
Mutation
Nucleic Acid Synthesis Inhibitors - pharmacology
Ribonucleotide Reductases - genetics
Ribonucleotide Reductases - metabolism
Viral Proteins - genetics
Viral Proteins - metabolism
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Summary:In Escherichia coli ribonucleotide reduction is catalyzed by two separate enzymes during aerobic and anaerobic growth. The aerobic enzyme is coded by the nrdAB genes, the anaerobic enzyme by nrdDG. We now show that knock-out mutants of either nrdD or nrdG cannot grow during strict anaerobiosis, achieved by inclusion of sodium sulfide in the medium. Interestingly, these mutants grow well under microaerophilic conditions by overproducing the aerobic enzyme. Under such conditions wild-type bacteria turn off nrdAB and switch on nrdDG.
ISSN:0006-291X
DOI:10.1006/bbrc.1996.1778