Evaluation of active matrix metalloproteinase-8 (aMMP-8) chair-side test as a diagnostic biomarker in the staging of periodontal diseases

• Published in: Archives of oral biology 2021-04, Vol.124, p.104955-104955, Article 104955
• Main Authors: Öztürk, V. Özgen, Emingil, Gülnur, Umeizudike, Kehinde, Tervahartiala, Taina, Gieselmann, Dirk-Rolf, Maier, Kurt, Köse, Timur, Sorsa, Timo, Alassiri, Saeed
• Format: Article
• Language: English
• Subjects: Biomarkers - analysis; Chair-side test; Cross-Sectional Studies; Gingival crevicular fluid; Gingival Crevicular Fluid - chemistry; Gingivitis - diagnosis; Humans; Matrix Metalloproteinase 8; Periodontal diseases; Periodontal Diseases - diagnosis
• ISSN: 0003-9969; 1879-1506
• DOI: 10.1016/j.archoralbio.2020.104955

•GCF aMMP-8 chairside test sensitivity was 83.9 % and 79.2 % for specificity.•Stage IV periodontitis group had higher GCF aMMP-8 levels than the other groups.•Salivary aMMP-8 levels of P-Stage IV, III and gingivitis groups were higher than healthy group.•Low aMMP-8 levels but not total/latent MMP-8 clearly indicates periodontal health.•aMMP-8 is useful diagnostic biomarker for early detection in the new periodontitis classification. There is a need for a reliable complementary diagnostic tool that ideally helps to screen, differentiate sites, activities of and predict future periodontal tissue destruction. The purpose of this cross-sectional study was to investigate the screening and prevention potential of the chair-side/point-of-care (PoC) diagnostic test of salivary active matrix metalloproteinase-8 (aMMP-8) levels at different stages of periodontal disease and periodontal health. 80 individuals were included in this study; 18 with periodontitis stage 3 (P-Stage III), 19 with periodontitis stage-4 (P-Stage IV), 21 with gingivitis, and 22 with clinically healthy periodontium (H). The aMMP-8 levels in GCF and saliva were analyzed by chairside point-of-care aMMP-8 lateral flow immunotest and also by a time-resolved immunofluorescence assay (IFMA). The sensitivity of the chair-side/PoC test was 83.9 % while specificity was 79.2 %. The aMMP-8 IFMA levels in GCF were significantly higher in P-Stage IV group than P-Stage III, gingivitis and healthy groups (p = 0.01, p = 0.001, p = 0.00, respectively). Moreover, P-Stage III and gingivitis groups had significantly higher aMMP-8 IFMA levels than the healthy group (p