Cellular Trafficking of Glutathione Transferase M2-2 Between U373MG and SHSY-S7 Cells is Mediated by Exosomes

The enzyme glutathione transferase M2-2, expressed in human astrocytes, increases its expression in the presence of aminochrome and catalyzes the conjugation of aminochrome, preventing its toxic effects. Secretion of the enzyme glutathione transferase M2-2 from U373MG cells, used as a cellular model...

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Bibliographic Details
Published in:Neurotoxicity research 2021-04, Vol.39 (2), p.182-190
Main Authors: Valdes, Raúl, Armijo, Alicia, Muñoz, Patricia, Hultenby, Kjell, Hagg, Andres, Inzunza, Jose, Nalvarte, Ivan, Varshney, Mukesh, Mannervik, Bengt, Segura-Aguilar, Juan
Format: Article
Language:English
Subjects:
Aminochrome
Astrocytes
Biomedical and Life Sciences
Biomedicine
Cell Biology
Dopamine
Dopaminergic neurons
Exosomes
Medicin och hälsovetenskap
Neurobiology
Neurochemistry
Neurology
Neuroprotection
Neurosciences
Original Article
Parkinson´s disease
Pharmacology/Toxicology
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Summary:The enzyme glutathione transferase M2-2, expressed in human astrocytes, increases its expression in the presence of aminochrome and catalyzes the conjugation of aminochrome, preventing its toxic effects. Secretion of the enzyme glutathione transferase M2-2 from U373MG cells, used as a cellular model for astrocytes, has been reported, and the enzyme is taken up by neuroblastoma SYSH-S7 cells and provide protection against aminochrome. The present study provides evidence that glutathione transferase M2-2 is released in exosomes from U373MG cells, thereby providing a means for intercellular transport of the enzyme. With particular relevance to Parkinson disease and other degenerative conditions, we propose a new mechanism by which astrocytes may protect dopaminergic neurons against the endogenous neurotoxin aminochrome.
ISSN:1029-8428
1476-3524
1476-3524
DOI:10.1007/s12640-020-00327-5