Untargeted LC-MS Metabolomics for the Analysis of Micro-scaled Extracellular Metabolites from Hepatocytes

Metabolome analysis in micro physiological models is a challenge due to the low volume of the cell culture medium (CCM). Here, we report a LC-MS-based untargeted metabolomics protocol for the detection of hepatocyte extracellular metabolites from micro-scale samples of CCM. Using a single LC-MS meth...

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Bibliographic Details
Published in:Analytical Sciences 2021/07/10, Vol.37(7), pp.1049-1052
Main Authors: ABDALKADER, Rodi, CHALECKIS, Romanas, MEISTER, Isabel, ZHANG, Pei, WHEELOCK, Craig E., KAMEI, Ken-ichiro
Format: Article
Language:English
Subjects:
Analytical Chemistry
Cell culture
Chemistry
Chromatography, Liquid
extracellular metabolites
Hepatocyte
Hepatocytes
LC-MS
Medicin och hälsovetenskap
Metabolites
Metabolome
Metabolomics
Physiology
Tandem Mass Spectrometry
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Summary:Metabolome analysis in micro physiological models is a challenge due to the low volume of the cell culture medium (CCM). Here, we report a LC-MS-based untargeted metabolomics protocol for the detection of hepatocyte extracellular metabolites from micro-scale samples of CCM. Using a single LC-MS method we have detected 57 metabolites of which 27 showed >2-fold shifts after 72-hour incubation. We demonstrate that micro-scale CCM samples can be used for modelling micro-physiological temporal dynamics in metabolite intensities.
ISSN:0910-6340
1348-2246
1348-2246
DOI:10.2116/analsci.20N032