Anti-staphylococcal activity and mode of action of thioridazine photoproducts

• Published in: Scientific reports 2020-10, Vol.10 (1), p.18043-18043, Article 18043
• Main Authors: Tozar, Tatiana, Santos Costa, Sofia, Udrea, Ana-Maria, Nastasa, Viorel, Couto, Isabel, Viveiros, Miguel, Pascu, Mihail Lucian, Romanitan, Mihaela Oana
• Format: Article
• Language: English
• Subjects: 631/1647; 631/1647/48; 631/1647/527; 631/1647/527/1819; 631/1647/527/1820; 631/1647/527/2257; 631/326/22; 631/326/22/1434; Absorption spectroscopy; Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - radiation effects; Antibiotic resistance; Antibiotics; Antimicrobial activity; Antimicrobial agents; Antipsychotic Agents - pharmacology; Antipsychotic Agents - radiation effects; Bacteria; Ciprofloxacin; Drug Repositioning - methods; Drug resistance; Drug Resistance, Bacterial; Gram-positive bacteria; Humanities and Social Sciences; Infectious diseases; Irradiation; Laser radiation; Lasers; Methicillin; Methicillin-Resistant Staphylococcus aureus - drug effects; Mode of action; Molecular modelling; multidisciplinary; Penicillin; Science; Science (multidisciplinary); Solutions; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus infections; Surface tension; Thin layer chromatography; Thioridazine; Thioridazine - pharmacology; Thioridazine - radiation effects; Water
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-74752-z

Antibiotic resistance became an increasing risk for population health threatening our ability to fight infectious diseases. The objective of this study was to evaluate the activity of laser irradiated thioridazine (TZ) against clinically-relevant bacteria in view to fight antibiotic resistance. TZ in ultrapure water solutions was irradiated (1–240 min) with 266 nm pulsed laser radiation. Irradiated solutions were characterized by UV–Vis and FTIR absorption spectroscopy, thin layer chromatography, laser-induced fluorescence, and dynamic surface tension measurements. Molecular docking studies were made to evaluate the molecular mechanisms of photoproducts action against Staphylococcus aureus and MRSA. More general, solutions were evaluated for their antimicrobial and efflux inhibitory activity against a panel of bacteria of clinical relevance. We observed an enhanced antimicrobial activity of TZ photoproducts against Gram-positive bacteria. This was higher than ciprofloxacin effects for methicillin- and ciprofloxacin-resistant Staphylococcus aureus . Molecular docking showed the Penicillin-binding proteins PBP3 and PBP2a inhibition by sulforidazine as a possible mechanism of action against Staphylococcus aureus and MRSA strains, respectively. Irradiated TZ reveals possible advantages in the treatment of infectious diseases produced by antibiotic-resistant Gram-positive bacteria. TZ repurposing and its photoproducts, obtained by laser irradiation, show accelerated and low-costs of development if compared to chemical synthesis.