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Targeted metabolomics of CSF in healthy individuals and patients with secondary progressive multiple sclerosis using high-resolution mass spectrometry
Introduction Standardized commercial kits enable targeted metabolomics analysis and may thus provide an attractive complement to the more explorative approaches. The kits are typically developed for triple quadrupole mass spectrometers using serum and plasma. Objectives Here we measure the concentra...
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| Published in: | Metabolomics 2020, Vol.16 (2), p.26-26, Article 26 |
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| creator | Carlsson, Henrik Abujrais, Sandy Herman, Stephanie Khoonsari, Payam Emami Åkerfeldt, Torbjörn Svenningsson, Anders Burman, Joachim Kultima, Kim |
| description | Introduction
Standardized commercial kits enable targeted metabolomics analysis and may thus provide an attractive complement to the more explorative approaches. The kits are typically developed for triple quadrupole mass spectrometers using serum and plasma.
Objectives
Here we measure the concentrations of preselected metabolites in cerebrospinal fluid (CSF) using a kit developed for high-resolution mass spectrometry (HRMS). Secondarily, the study aimed to investigate metabolite alterations in patients with secondary progressive multiple sclerosis (SPMS) compared to controls.
Methods
We performed targeted metabolomics in human CSF on twelve SPMS patients and twelve age and sex-matched healthy controls using the Absolute IDQ-p400 kit (Biocrates Life Sciences AG) developed for HRMS. The extracts were analysed using two methods; liquid chromatography-mass spectrometry (LC-HRMS) and flow injection analysis-MS (FIA-HRMS).
Results
Out of 408 targeted metabolites, 196 (48%) were detected above limit of detection and 35 were absolutely quantified. Metabolites analyzed using LC-HRMS had a median coefficient of variation (CV) of 3% and 2.5% between reinjections the same day and after prolonged storage, respectively. The corresponding results for the FIA-HRMS were a median CV of 27% and 21%, respectively. We found significantly (p |
| doi_str_mv | 10.1007/s11306-020-1648-5 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_swepu</sourceid><recordid>TN_cdi_swepub_primary_oai_swepub_ki_se_473510</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2354735581</sourcerecordid><originalsourceid>FETCH-LOGICAL-c595t-c2d36b5909ab6ee712d8dc1fc354b856bafad84d37c8885dec7b2e9d7757e8463</originalsourceid><addsrcrecordid>eNp1ksFu1DAQhiMEoqXwAFyQJS4cCNhOHNsXpGqhgFSJA4Wr5diziUsSB9tptS_C8-LVbrcUaU8Zxd8_43_8F8VLgt8RjPn7SEiFmxJTXJKmFiV7VJwSxquyEhI_PtSCnhTPYrzGuK4lx0-Lk4piRomQp8WfKx06SGDRCEm3fvCjMxH5NVp9v0BuQj3oIfWbXFp34-yih4j0ZNGsk4MpRXTrUo8iGD9ZHTZoDr4LEKO7ATQuQ3LzACiaAYKPLqIluqlDvev6MlN-WJLzExp1jCjOYFLw-R5h87x4ss6T4MX-e1b8uPh0tfpSXn77_HV1flkaJlkqDbVV0zKJpW4bAE6oFdaQtalY3QrWtHqtrahtxY0QglkwvKUgLeeMg6ib6qwod33jLcxLq-bgxuxCee3U_tevXIGqecUIzrw8ymfn9l50JyR1hQklVGTt26Paj-7nufKhU8uiasxryTL-YYdndgRr8rKDHh5OfHAyuV51_kZxTJhstt7e7BsE_3uBmNToooFh0BP4JSqal5RdMUEy-vo_9NovYcqb31KVpFjSOlNkR5n8ljHA-nAZgtU2kGoXSJUDqbaBVFsXr_51cVDcJTADdL-VfDR1EO5HH-_6F5rA8pc</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2353920924</pqid></control><display><type>article</type><title>Targeted metabolomics of CSF in healthy individuals and patients with secondary progressive multiple sclerosis using high-resolution mass spectrometry</title><source>Springer Nature:Jisc Collections:Springer Nature Read and Publish 2023-2025: Springer Reading List</source><creator>Carlsson, Henrik ; Abujrais, Sandy ; Herman, Stephanie ; Khoonsari, Payam Emami ; Åkerfeldt, Torbjörn ; Svenningsson, Anders ; Burman, Joachim ; Kultima, Kim</creator><creatorcontrib>Carlsson, Henrik ; Abujrais, Sandy ; Herman, Stephanie ; Khoonsari, Payam Emami ; Åkerfeldt, Torbjörn ; Svenningsson, Anders ; Burman, Joachim ; Kultima, Kim</creatorcontrib><description>Introduction
Standardized commercial kits enable targeted metabolomics analysis and may thus provide an attractive complement to the more explorative approaches. The kits are typically developed for triple quadrupole mass spectrometers using serum and plasma.
Objectives
Here we measure the concentrations of preselected metabolites in cerebrospinal fluid (CSF) using a kit developed for high-resolution mass spectrometry (HRMS). Secondarily, the study aimed to investigate metabolite alterations in patients with secondary progressive multiple sclerosis (SPMS) compared to controls.
Methods
We performed targeted metabolomics in human CSF on twelve SPMS patients and twelve age and sex-matched healthy controls using the Absolute IDQ-p400 kit (Biocrates Life Sciences AG) developed for HRMS. The extracts were analysed using two methods; liquid chromatography-mass spectrometry (LC-HRMS) and flow injection analysis-MS (FIA-HRMS).
Results
Out of 408 targeted metabolites, 196 (48%) were detected above limit of detection and 35 were absolutely quantified. Metabolites analyzed using LC-HRMS had a median coefficient of variation (CV) of 3% and 2.5% between reinjections the same day and after prolonged storage, respectively. The corresponding results for the FIA-HRMS were a median CV of 27% and 21%, respectively. We found significantly (p < 0.05) elevated levels of glycine, asymmetric dimethylarginine (ADMA), glycerophospholipid PC-O (34:0) and sum of hexoses in SPMS patients compared to controls.
Conclusion
The Absolute IDQ-p400 kit could successfully be used for quantifying targeted metabolites in the CSF. Metabolites quantified using LC-HRMS showed superior reproducibility compared to FIA-HRMS.</description><identifier>ISSN: 1573-3882</identifier><identifier>ISSN: 1573-3890</identifier><identifier>EISSN: 1573-3890</identifier><identifier>DOI: 10.1007/s11306-020-1648-5</identifier><identifier>PMID: 32052189</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Cerebrospinal fluid ; Chromatography, Liquid ; Cohort Studies ; Developmental Biology ; Female ; Flow Injection Analysis ; Glycine ; High-resolution mass spectrometry ; Humans ; Life Sciences ; Liquid chromatography ; Male ; Mass Spectrometry ; Mass spectroscopy ; Medicin och hälsovetenskap ; Metabolites ; Metabolomics ; Middle Aged ; Molecular Medicine ; Multiple sclerosis ; Multiple Sclerosis - cerebrospinal fluid ; Multiple Sclerosis - metabolism ; Multiple Sclerosis - pathology ; Original ; Original Article ; Scientific imaging ; Secondary progressive multiple sclerosis ; Targeted metabolomics</subject><ispartof>Metabolomics, 2020, Vol.16 (2), p.26-26, Article 26</ispartof><rights>The Author(s) 2020</rights><rights>Metabolomics is a copyright of Springer, (2020). All Rights Reserved. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c595t-c2d36b5909ab6ee712d8dc1fc354b856bafad84d37c8885dec7b2e9d7757e8463</citedby><cites>FETCH-LOGICAL-c595t-c2d36b5909ab6ee712d8dc1fc354b856bafad84d37c8885dec7b2e9d7757e8463</cites><orcidid>0000-0002-0680-1410</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32052189$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-407495$$DView record from Swedish Publication Index$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:143012128$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Carlsson, Henrik</creatorcontrib><creatorcontrib>Abujrais, Sandy</creatorcontrib><creatorcontrib>Herman, Stephanie</creatorcontrib><creatorcontrib>Khoonsari, Payam Emami</creatorcontrib><creatorcontrib>Åkerfeldt, Torbjörn</creatorcontrib><creatorcontrib>Svenningsson, Anders</creatorcontrib><creatorcontrib>Burman, Joachim</creatorcontrib><creatorcontrib>Kultima, Kim</creatorcontrib><title>Targeted metabolomics of CSF in healthy individuals and patients with secondary progressive multiple sclerosis using high-resolution mass spectrometry</title><title>Metabolomics</title><addtitle>Metabolomics</addtitle><addtitle>Metabolomics</addtitle><description>Introduction
Standardized commercial kits enable targeted metabolomics analysis and may thus provide an attractive complement to the more explorative approaches. The kits are typically developed for triple quadrupole mass spectrometers using serum and plasma.
Objectives
Here we measure the concentrations of preselected metabolites in cerebrospinal fluid (CSF) using a kit developed for high-resolution mass spectrometry (HRMS). Secondarily, the study aimed to investigate metabolite alterations in patients with secondary progressive multiple sclerosis (SPMS) compared to controls.
Methods
We performed targeted metabolomics in human CSF on twelve SPMS patients and twelve age and sex-matched healthy controls using the Absolute IDQ-p400 kit (Biocrates Life Sciences AG) developed for HRMS. The extracts were analysed using two methods; liquid chromatography-mass spectrometry (LC-HRMS) and flow injection analysis-MS (FIA-HRMS).
Results
Out of 408 targeted metabolites, 196 (48%) were detected above limit of detection and 35 were absolutely quantified. Metabolites analyzed using LC-HRMS had a median coefficient of variation (CV) of 3% and 2.5% between reinjections the same day and after prolonged storage, respectively. The corresponding results for the FIA-HRMS were a median CV of 27% and 21%, respectively. We found significantly (p < 0.05) elevated levels of glycine, asymmetric dimethylarginine (ADMA), glycerophospholipid PC-O (34:0) and sum of hexoses in SPMS patients compared to controls.
Conclusion
The Absolute IDQ-p400 kit could successfully be used for quantifying targeted metabolites in the CSF. Metabolites quantified using LC-HRMS showed superior reproducibility compared to FIA-HRMS.</description><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Cerebrospinal fluid</subject><subject>Chromatography, Liquid</subject><subject>Cohort Studies</subject><subject>Developmental Biology</subject><subject>Female</subject><subject>Flow Injection Analysis</subject><subject>Glycine</subject><subject>High-resolution mass spectrometry</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Liquid chromatography</subject><subject>Male</subject><subject>Mass Spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medicin och hälsovetenskap</subject><subject>Metabolites</subject><subject>Metabolomics</subject><subject>Middle Aged</subject><subject>Molecular Medicine</subject><subject>Multiple sclerosis</subject><subject>Multiple Sclerosis - cerebrospinal fluid</subject><subject>Multiple Sclerosis - metabolism</subject><subject>Multiple Sclerosis - pathology</subject><subject>Original</subject><subject>Original Article</subject><subject>Scientific imaging</subject><subject>Secondary progressive multiple sclerosis</subject><subject>Targeted metabolomics</subject><issn>1573-3882</issn><issn>1573-3890</issn><issn>1573-3890</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp1ksFu1DAQhiMEoqXwAFyQJS4cCNhOHNsXpGqhgFSJA4Wr5diziUsSB9tptS_C8-LVbrcUaU8Zxd8_43_8F8VLgt8RjPn7SEiFmxJTXJKmFiV7VJwSxquyEhI_PtSCnhTPYrzGuK4lx0-Lk4piRomQp8WfKx06SGDRCEm3fvCjMxH5NVp9v0BuQj3oIfWbXFp34-yih4j0ZNGsk4MpRXTrUo8iGD9ZHTZoDr4LEKO7ATQuQ3LzACiaAYKPLqIluqlDvev6MlN-WJLzExp1jCjOYFLw-R5h87x4ss6T4MX-e1b8uPh0tfpSXn77_HV1flkaJlkqDbVV0zKJpW4bAE6oFdaQtalY3QrWtHqtrahtxY0QglkwvKUgLeeMg6ib6qwod33jLcxLq-bgxuxCee3U_tevXIGqecUIzrw8ymfn9l50JyR1hQklVGTt26Paj-7nufKhU8uiasxryTL-YYdndgRr8rKDHh5OfHAyuV51_kZxTJhstt7e7BsE_3uBmNToooFh0BP4JSqal5RdMUEy-vo_9NovYcqb31KVpFjSOlNkR5n8ljHA-nAZgtU2kGoXSJUDqbaBVFsXr_51cVDcJTADdL-VfDR1EO5HH-_6F5rA8pc</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>Carlsson, Henrik</creator><creator>Abujrais, Sandy</creator><creator>Herman, Stephanie</creator><creator>Khoonsari, Payam Emami</creator><creator>Åkerfeldt, Torbjörn</creator><creator>Svenningsson, Anders</creator><creator>Burman, Joachim</creator><creator>Kultima, Kim</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>ACNBI</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>DF2</scope><scope>ZZAVC</scope><orcidid>https://orcid.org/0000-0002-0680-1410</orcidid></search><sort><creationdate>2020</creationdate><title>Targeted metabolomics of CSF in healthy individuals and patients with secondary progressive multiple sclerosis using high-resolution mass spectrometry</title><author>Carlsson, Henrik ; Abujrais, Sandy ; Herman, Stephanie ; Khoonsari, Payam Emami ; Åkerfeldt, Torbjörn ; Svenningsson, Anders ; Burman, Joachim ; Kultima, Kim</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c595t-c2d36b5909ab6ee712d8dc1fc354b856bafad84d37c8885dec7b2e9d7757e8463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell Biology</topic><topic>Cerebrospinal fluid</topic><topic>Chromatography, Liquid</topic><topic>Cohort Studies</topic><topic>Developmental Biology</topic><topic>Female</topic><topic>Flow Injection Analysis</topic><topic>Glycine</topic><topic>High-resolution mass spectrometry</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Liquid chromatography</topic><topic>Male</topic><topic>Mass Spectrometry</topic><topic>Mass spectroscopy</topic><topic>Medicin och hälsovetenskap</topic><topic>Metabolites</topic><topic>Metabolomics</topic><topic>Middle Aged</topic><topic>Molecular Medicine</topic><topic>Multiple sclerosis</topic><topic>Multiple Sclerosis - cerebrospinal fluid</topic><topic>Multiple Sclerosis - metabolism</topic><topic>Multiple Sclerosis - pathology</topic><topic>Original</topic><topic>Original Article</topic><topic>Scientific imaging</topic><topic>Secondary progressive multiple sclerosis</topic><topic>Targeted metabolomics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carlsson, Henrik</creatorcontrib><creatorcontrib>Abujrais, Sandy</creatorcontrib><creatorcontrib>Herman, Stephanie</creatorcontrib><creatorcontrib>Khoonsari, Payam Emami</creatorcontrib><creatorcontrib>Åkerfeldt, Torbjörn</creatorcontrib><creatorcontrib>Svenningsson, Anders</creatorcontrib><creatorcontrib>Burman, Joachim</creatorcontrib><creatorcontrib>Kultima, Kim</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SWEPUB Uppsala universitet full text</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Freely available online</collection><collection>SWEPUB Uppsala universitet</collection><collection>SwePub Articles full text</collection><jtitle>Metabolomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carlsson, Henrik</au><au>Abujrais, Sandy</au><au>Herman, Stephanie</au><au>Khoonsari, Payam Emami</au><au>Åkerfeldt, Torbjörn</au><au>Svenningsson, Anders</au><au>Burman, Joachim</au><au>Kultima, Kim</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Targeted metabolomics of CSF in healthy individuals and patients with secondary progressive multiple sclerosis using high-resolution mass spectrometry</atitle><jtitle>Metabolomics</jtitle><stitle>Metabolomics</stitle><addtitle>Metabolomics</addtitle><date>2020</date><risdate>2020</risdate><volume>16</volume><issue>2</issue><spage>26</spage><epage>26</epage><pages>26-26</pages><artnum>26</artnum><issn>1573-3882</issn><issn>1573-3890</issn><eissn>1573-3890</eissn><abstract>Introduction
Standardized commercial kits enable targeted metabolomics analysis and may thus provide an attractive complement to the more explorative approaches. The kits are typically developed for triple quadrupole mass spectrometers using serum and plasma.
Objectives
Here we measure the concentrations of preselected metabolites in cerebrospinal fluid (CSF) using a kit developed for high-resolution mass spectrometry (HRMS). Secondarily, the study aimed to investigate metabolite alterations in patients with secondary progressive multiple sclerosis (SPMS) compared to controls.
Methods
We performed targeted metabolomics in human CSF on twelve SPMS patients and twelve age and sex-matched healthy controls using the Absolute IDQ-p400 kit (Biocrates Life Sciences AG) developed for HRMS. The extracts were analysed using two methods; liquid chromatography-mass spectrometry (LC-HRMS) and flow injection analysis-MS (FIA-HRMS).
Results
Out of 408 targeted metabolites, 196 (48%) were detected above limit of detection and 35 were absolutely quantified. Metabolites analyzed using LC-HRMS had a median coefficient of variation (CV) of 3% and 2.5% between reinjections the same day and after prolonged storage, respectively. The corresponding results for the FIA-HRMS were a median CV of 27% and 21%, respectively. We found significantly (p < 0.05) elevated levels of glycine, asymmetric dimethylarginine (ADMA), glycerophospholipid PC-O (34:0) and sum of hexoses in SPMS patients compared to controls.
Conclusion
The Absolute IDQ-p400 kit could successfully be used for quantifying targeted metabolites in the CSF. Metabolites quantified using LC-HRMS showed superior reproducibility compared to FIA-HRMS.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>32052189</pmid><doi>10.1007/s11306-020-1648-5</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-0680-1410</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Biochemistry Biomedical and Life Sciences Biomedicine Cell Biology Cerebrospinal fluid Chromatography, Liquid Cohort Studies Developmental Biology Female Flow Injection Analysis Glycine High-resolution mass spectrometry Humans Life Sciences Liquid chromatography Male Mass Spectrometry Mass spectroscopy Medicin och hälsovetenskap Metabolites Metabolomics Middle Aged Molecular Medicine Multiple sclerosis Multiple Sclerosis - cerebrospinal fluid Multiple Sclerosis - metabolism Multiple Sclerosis - pathology Original Original Article Scientific imaging Secondary progressive multiple sclerosis Targeted metabolomics |
| title | Targeted metabolomics of CSF in healthy individuals and patients with secondary progressive multiple sclerosis using high-resolution mass spectrometry |
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