Physical activity may drive healthy microvascular ageing via downregulation of p66 Shc

Narrower retinal arterioles and wider venules are linked to adverse cardiovascular outcomes. The mitochondrial adaptor p66 is a major source of ageing-induced generation of reactive oxygen species. Promoter DNA methylation inhibits p66 gene transcription. This cross-sectional study was designed to i...

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Published in:European journal of preventive cardiology 2020-01, Vol.27 (2), p.168-176
Main Authors: Streese, Lukas, Khan, Abdul W, Deiseroth, Arne, Hussain, Shafaat, Suades, Rosa, Tiaden, Andre, Kyburz, Diego, Hanssen, Henner, Cosentino, Francesco
Format: Article
Language:English
Subjects:
Age Factors
Aged
Arterioles - physiology
Biomarkers - blood
Cross-Sectional Studies
DNA Methylation
Down-Regulation
Exercise
Female
Healthy Aging - blood
Healthy Aging - genetics
Humans
Male
Middle Aged
Oxidative Stress
Retinal Vessels - physiology
Sedentary Behavior
Src Homology 2 Domain-Containing, Transforming Protein 1 - blood
Src Homology 2 Domain-Containing, Transforming Protein 1 - genetics
Tyrosine - analogs & derivatives
Tyrosine - blood
Venules - physiology
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Summary:Narrower retinal arterioles and wider venules are linked to adverse cardiovascular outcomes. The mitochondrial adaptor p66 is a major source of ageing-induced generation of reactive oxygen species. Promoter DNA methylation inhibits p66 gene transcription. This cross-sectional study was designed to investigate the link between physical activity, retinal vessel diameters and p66 expression in active and sedentary ageing subjects. Altogether 158 subjects were included in the study (mean age 59.4 ± 7.0 years). Thirty-eight subjects were healthy active, 36 were healthy sedentary and 84 were sedentary with ≥2 cardiovascular risk factors. Retinal arteriolar and venular diameters were measured by means of a retinal vessel analyser. As a marker of oxidative stress, plasma 3-nitrotyrosine was determined by enzyme-linked immunosorbent assay. Gene expression of p66 and DNA methylation were assessed in mononuclear cells by real-time quantitative polymerase chain reaction and methylated-DNA capture (MethylMiner Enrichment kit) coupled with quantitative polymerase chain reaction, respectively. Wider retinal arterioles (179 ± 14 vs 172 ± 11 and 171 ± 14 µm;  
ISSN:2047-4873
2047-4881
DOI:10.1177/2047487319880367