Pro-Angiogenic Macrophage Phenotype to Promote Myocardial Repair

• Published in: Journal of the American College of Cardiology 2019-06, Vol.73 (23), p.2990-3002
• Main Authors: Ferraro, Bartolo, Leoni, Giovanna, Hinkel, Rabea, Ormanns, Steffen, Paulin, Nicole, Ortega-Gomez, Almudena, Viola, Joana R, de Jong, Renske, Bongiovanni, Dario, Bozoglu, Tarik, Maas, Sanne L, D'Amico, Michele, Kessler, Thorsten, Zeller, Tanja, Hristov, Michael, Reutelingsperger, Chris, Sager, Hendrik B, Döring, Yvonne, Nahrendorf, Matthias, Kupatt, Christian, Soehnlein, Oliver
• Format: Article
• Language: English
• Subjects: Angiogenesis; Animals; Annexin A1 - deficiency; Annexin A1 - genetics; Bone marrow; Bone marrow transplantation; Cardiology; Cardiovascular disease; Cell adhesion & migration; Coronary artery; Coronary vessels; Experiments; Female; Flow cytometry; Flox; Fluorescence; Genotype & phenotype; Growth factors; Heart; Heart attacks; Inflammation; Ischemia; Macrophages; Macrophages - physiology; Mice; Mice, Inbred C57BL; Mice, Knockout; Myocardial infarction; Myocardial Infarction - genetics; Myocardial Infarction - metabolism; Myocardium - metabolism; Myocardium - pathology; Nanoparticles; Neovascularization, Physiologic - physiology; Neutrophils; Ostomy; Peptides; Phenotype; Phenotypes; Regeneration; Repair; Studies; Vascular endothelial growth factor; Vascularization; Viruses
• ISSN: 0735-1097; 1558-3597
• DOI: 10.1016/j.jacc.2019.03.503

Heart failure following myocardial infarction (MI) remains one of the major causes of death worldwide, and its treatment is a crucial challenge of cardiovascular medicine. An attractive therapeutic strategy is to stimulate endogenous mechanisms of myocardial regeneration. This study evaluates the potential therapeutic treatment with annexin A1 (AnxA1) to induce cardiac repair after MI. AnxA1 knockout (AnxA1 ) and wild-type mice underwent MI induced by ligation of the left anterior descending coronary artery. Cardiac functionality was assessed by longitudinal echocardiographic measurements. Histological, fluorescence-activated cell sorting, dot blot analysis, and in vitro/ex vivo studies were used to assess the myocardial neovascularization, macrophage content, and activity in response to AnxA1. AnxA1 mice showed a reduced cardiac functionality and an expansion of proinflammatory macrophages in the ischemic area. Cardiac macrophages from AnxA1 mice exhibited a dramatically reduced ability to release the proangiogenic mediator vascular endothelial growth factor (VEGF)-A. However, AnxA1 treatment enhanced VEGF-A release from cardiac macrophages, and its delivery in vivo markedly improved cardiac performance. The positive effect of AnxA1 treatment on cardiac performance was abolished in wild-type mice transplanted with bone marrow derived from Cx cr1cre Vegf or in mice depleted of macrophages. Similarly, cardioprotective effects of AnxA1 were obtained in pigs in which full-length AnxA1 was overexpressed by use of a cardiotropic adeno-associated virus. AnxA1 has a direct action on cardiac macrophage polarization toward a pro-angiogenic, reparative phenotype. AnxA1 stimulated cardiac macrophages to release high amounts of VEGF-A, thus inducing neovascularization and cardiac repair.