Probiotic intervention influences the salivary levels of Matrix Metalloproteinase (MMP)-9 and Tissue Inhibitor of metalloproteinases (TIMP)-1 in healthy adults

• Published in: Archives of oral biology 2018-01, Vol.85, p.58-63
• Main Authors: Jäsberg, Heli, Tervahartiala, Taina, Sorsa, Timo, Söderling, Eva, Haukioja, Anna
• Format: Article
• Language: English
• Subjects: Adult; Bifidobacterium animalis; Bifidobacterium animalis subsp. lactis BB-12; Dental Plaque Index; Dentistry; Enzyme-Linked Immunosorbent Assay; Female; Healthy Volunteers; Humans; Lactobacillus rhamnosus; Lactobacillus rhamnosus GG; Male; Matrix metalloproteinase (MMP); Matrix Metalloproteinase 8 - metabolism; Matrix Metalloproteinase 9 - metabolism; Medicin och hälsovetenskap; Periodontal Index; Probiotic; Probiotics - pharmacology; Saliva; Saliva - chemistry; Saliva - microbiology; Tissue Inhibitor of Metalloproteinase-1 - metabolism; Tissue inhibitor of metalloproteinases (TIMP)
• ISSN: 0003-9969; 1879-1506; 1879-1506
• DOI: 10.1016/j.archoralbio.2017.10.003

•Probiotic BB-12 and LGG affected salivary MMP-9 and TIMP-1 levels in healthy adults.•Salivary MMP-8 levels remained unaffected after probiotic intervention.•Salivary MMP-9 levels and gingival index correlated.•Increased MMP-9 and decreased TIMP-1 indicate immunomodulatory effect of probiotics.•Increased MMP-9 may indicate the defensive potential of matrix metalloproteinases. To study the effect of orally administered Bifidobacterium animalis subsp. lactis BB-12 and Lactobacillus rhamnosus GG on the salivary levels of Matrix Metalloproteinases (MMP)-8, MMP-9 and of Tissue Inhibitor of Metalloproteinases (TIMP)-1 in healthy adults. Furthermore, the correlations between MMP-8, MMP-9 and TIMP-1 and plaque and gingival indices, salivary mutans streptococci and lactobacilli counts, and stimulated saliva secretion rate were analysed. The salivary samples originated from a randomized controlled trial where healthy student volunteers consumed probiotic or placebo lozenges twice a day for four weeks. The saliva samples were collected and clinical parameters measured at the baseline and at the end of the original study. For this study, the salivary levels of MMP-8, MMP-9 and TIMP-1 were analysed with immunofluorometric assay (IFMA) and enzyme-linked immunosorbent assay (ELISA). In the probiotic group (n=29), salivary MMP-9 levels increased (p