Generation of human iPS cell line CTL07-II from human fibroblasts, under defined and xeno-free conditions

CTL07-II is a healthy feeder-free and characterized human induced pluripotent stem (iPS) cell line. Cultured under xeno-free and defined conditions. The line is generated from healthy human fibroblasts with non-integrating Sendai virus vectors encoding the four Yamanaka factors, OCT4, SOX2, KLF4 and...

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Bibliographic Details
Published in:Stem cell research 2016-11, Vol.17 (3), p.474-478
Main Authors: Kele, Malin, Day, Kelly, Rönnholm, Harriet, Schuster, Jens, Dahl, Niklas, Falk, Anna
Format: Article
Language:English
Subjects:
Cell Differentiation
Cell Line
Cellular Reprogramming
Culture Media - chemistry
Embryoid Bodies - cytology
Embryoid Bodies - metabolism
Fibroblasts - cytology
Genetic Vectors - genetics
Genetic Vectors - metabolism
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Karyotype
Male
Medicin och hälsovetenskap
Microscopy, Fluorescence
Sendai virus - genetics
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:CTL07-II is a healthy feeder-free and characterized human induced pluripotent stem (iPS) cell line. Cultured under xeno-free and defined conditions. The line is generated from healthy human fibroblasts with non-integrating Sendai virus vectors encoding the four Yamanaka factors, OCT4, SOX2, KLF4 and cMYC. The generated iPS cells are free from reprogramming vectors and their purity, karyotypic stability and pluripotent capacity is confirmed.
ISSN:1873-5061
1876-7753
1876-7753
DOI:10.1016/j.scr.2016.09.028