Rapid Bedside Inactivation of Ebola Virus for Safe Nucleic Acid Tests

• Published in: Journal of clinical microbiology 2016-10, Vol.54 (10), p.2521-2529
• Main Authors: Rosenstierne, Maiken Worsøe, Karlberg, Helen, Bragstad, Karoline, Lindegren, Gunnel, Stoltz, Malin Lundahl, Salata, Cristiano, Kran, Anne-Marte Bakken, Dudman, Susanne Gjeruldsen, Mirazimi, Ali, Fomsgaard, Anders
• Format: Article
• Language: English
• Subjects: Disinfection - methods; Ebolavirus; Hemorrhagic Fever, Ebola - diagnosis; Hemorrhagic Fever, Ebola - virology; Humans; Medicin och hälsovetenskap; Point-of-Care Systems; RNA, Viral - isolation & purification; Specimen Handling - methods; Temperature; Time Factors; Virology; Virus Inactivation
• ISSN: 0095-1137; 1098-660X; 1098-660X
• DOI: 10.1128/JCM.00346-16

Rapid bedside inactivation of Ebola virus would be a solution for the safety of medical and technical staff, risk containment, sample transport, and high-throughput or rapid diagnostic testing during an outbreak. We show that the commercially available Magna Pure lysis/binding buffer used for nucleic acid extraction inactivates Ebola virus. A rapid bedside inactivation method for nucleic acid tests is obtained by simply adding Magna Pure lysis/binding buffer directly into vacuum blood collection EDTA tubes using a thin needle and syringe prior to sampling. The ready-to-use inactivation vacuum tubes are stable for more than 4 months, and Ebola virus RNA is preserved in the Magna Pure lysis/binding buffer for at least 5 weeks independent of the storage temperature. We also show that Ebola virus RNA can be manually extracted from Magna Pure lysis/binding buffer-inactivated samples using the QIAamp viral RNA minikit. We present an easy and convenient method for bedside inactivation using available blood collection vacuum tubes and reagents. We propose to use this simple method for fast, safe, and easy bedside inactivation of Ebola virus for safe transport and routine nucleic acid detection.