Methods for the characterization of stress granules in virus infected cells

•Stress granules are induced as a cellular defence against virus infection.•We discuss methods for the detection of viral and cellular proteins and RNA in SGs.•In addition, we describe a surrogate in vitro assay for SG formation. Stress granules are induced in many different viral infections, and in...

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Bibliographic Details
Published in:Methods (San Diego, Calif.) Calif.), 2015-11, Vol.90, p.57-64
Main Authors: Panas, Marc D., Kedersha, Nancy, McInerney, Gerald M.
Format: Article
Language:English
Subjects:
Cell biology
Host-Pathogen Interactions
Medicin och hälsovetenskap
Microscopy - methods
Review
Ribonucleoproteins - chemistry
Ribonucleoproteins - metabolism
RNA, Viral - analysis
Signaling
Stress granules
Stress, Physiological
Translation
Viral Proteins - analysis
Virology - methods
Virus
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Summary:•Stress granules are induced as a cellular defence against virus infection.•We discuss methods for the detection of viral and cellular proteins and RNA in SGs.•In addition, we describe a surrogate in vitro assay for SG formation. Stress granules are induced in many different viral infections, and in turn are inhibited by the expression of viral proteins or RNAs. It is therefore evident that these bodies are not compatible with efficient viral replication, but the mechanism by which they act to restrict viral gene expression or genome replication is not yet understood. This article discusses a number of methods that can be employed to gain a more complete understanding of the relationship between cellular SGs and viral RNA and protein synthesis in cells infected with diverse viruses.
ISSN:1046-2023
1095-9130
1095-9130
DOI:10.1016/j.ymeth.2015.04.009