No difference in small bowel microbiota between patients with irritable bowel syndrome and healthy controls

Several studies have indicated that colonic microbiota may exhibit important differences between patients with irritable bowel syndrome (IBS) and healthy controls. Less is known about the microbiota of the small bowel. We used massive parallel sequencing to explore the composition of small bowel muc...

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Bibliographic Details
Published in:Scientific reports 2015-02, Vol.5 (1), p.8508-8508, Article 8508
Main Authors: Dlugosz, Aldona, Winckler, Björn, Lundin, Elin, Zakikhany, Katherina, Sandström, Gunnar, Ye, Weimin, Engstrand, Lars, Lindberg, Greger
Format: Article
Language:English
Subjects:
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Abundance
Adolescent
Adult
Biodiversity
Case-Control Studies
Classification
Cluster Analysis
Effectiveness
Female
Genera
Humanities and Social Sciences
Humans
Intestine, Small - microbiology
Irritable bowel syndrome
Irritable Bowel Syndrome - etiology
Jejunum
Male
Medicin och hälsovetenskap
Metagenome
Microbiota
Middle Aged
Mucosa
multidisciplinary
Principal Component Analysis
Science
Small intestine
Young Adult
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Summary:Several studies have indicated that colonic microbiota may exhibit important differences between patients with irritable bowel syndrome (IBS) and healthy controls. Less is known about the microbiota of the small bowel. We used massive parallel sequencing to explore the composition of small bowel mucosa-associated microbiota in patients with IBS and healthy controls. We analysed capsule biopsies from the jejunum of 35 patients (26 females) with IBS aged 18-(36)-57 years and 16 healthy volunteers (11 females) aged 20-(32)-48 years. Sequences were analysed based on taxonomic classification. The phyla with the highest total abundance across all samples were: Firmicutes (43%), Proteobacteria (23%), Bacteroidetes (15%), Actinobacteria (9.3%) and Fusobacteria (7.0%). The most abundant genera were: Streptococcus (19%), Veillonella (13%), Prevotella (12%), Rothia (6.4%), Haemophilus (5.7%), Actinobacillus (5.5%), Escherichia (4.6%) and Fusobacterium (4.3%). We found no difference among major phyla or genera between patients with IBS and controls. We identified a cluster of samples in the small bowel microbiota dominated by Prevotella , which may represent a common enterotype of the upper small intestine. The remaining samples formed a gradient, dominated by Streptococcus at one end and Escherichia at the other.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep08508