Immunohistochemical profile of ductal adenocarcinoma of the prostate

• Published in: Virchows Archiv : an international journal of pathology 2014-11, Vol.465 (5), p.559-565
• Main Authors: Seipel, Amanda H., Samaratunga, Hemamali, Delahunt, Brett, Wiklund, Fredrik, Wiklund, Peter, Lindberg, Johan, Grönberg, Henrik, Egevad, Lars
• Format: Article
• Language: English
• Subjects: Adenocarcinoma - pathology; Adenocarcinoma - physiopathology; Biomarkers, Tumor; Carcinoma, Acinar Cell - pathology; Carcinoma, Acinar Cell - physiopathology; Cyclin-Dependent Kinase Inhibitor p16 - biosynthesis; Estrogens; Humans; Immunohistochemistry; Ki-67 Antigen - biosynthesis; Male; Medicin och hälsovetenskap; Medicine; Medicine & Public Health; Original Article; Pathology; Prostate - pathology; Prostate cancer; Prostatic Neoplasms - pathology; Prostatic Neoplasms - physiopathology; Protein Array Analysis; Tumor Suppressor Protein p53 - biosynthesis
• ISSN: 0945-6317; 1432-2307; 1432-2307
• DOI: 10.1007/s00428-014-1636-0

Ductal adenocarcinoma of the prostate (DAC) is considered to be an aggressive subtype of prostate cancer with greater risk of progression than acinar adenocarcinoma (AC). It has been debated whether DAC is a distinct subtype or a morphological variant of AC. Our aim was to examine the protein expression of DAC and to compare the results with AC. A tissue microarray was constructed from 60 DAC and 46 AC matched by Gleason score. The slides were stained for 28 immunomarkers (estrogen, progesterone and androgen receptor, prolactin, PSA, prostein, PSMA, PSAP, CDX2, lysozyme, villin, monoclonal CEA, CK7, CK20, HMWCK, p63, p504s, c-myc, EGFR, Ki-67, p16, p21, p27, p53, PTEN, ERG, PAX-2, and PAX-8). HMWCK was positive in 8.5 % of DAC, but negative in all cases of AC ( p  = 0.045). p16 was positive in 53.3 % of DAC and in 26.1 % of AC ( p  = 0.005). p53 was positive in 42.4 % of DAC and 26.7 % of AC ( p  = 0.031). A distinct patchy positivity of CK20 was seen in 23.7 % of DAC, and this pattern was also seen in 9.1 % of AC ( p  = 0.047). Villin was positive in 3.4 % of DAC while expression was negative in AC. Ki-67 labeling index was significantly higher in DAC than in AC (mean 9.2 % [95 % CI 6.4–12.0] and 2.6 % [1.9–3.4], p