Deep sequencing extends the diversity of human papillomaviruses in human skin

Most viruses in human skin are known to be human papillomaviruses (HPVs). Previous sequencing of skin samples has identified 273 different cutaneous HPV types, including 47 previously unknown types. In the present study, we wished to extend prior studies using deeper sequencing. This deeper sequenci...

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Bibliographic Details
Published in:Scientific reports 2014-07, Vol.4 (1), p.5807-5807, Article 5807
Main Authors: Bzhalava, Davit, Mühr, Laila Sara Arroyo, Lagheden, Camilla, Ekström, Johanna, Forslund, Ola, Dillner, Joakim, Hultin, Emilie
Format: Article
Language:English
Subjects:
49/23
631/326/596/2560
692/308/2778
Alphapapillomavirus - genetics
Annan klinisk medicin
Basic Medicine
Bayes Theorem
Carcinoma, Squamous Cell - virology
Clinical Medicine
DNA, Viral - genetics
Genetic Variation
Genomes
High-Throughput Nucleotide Sequencing
Human papillomavirus
Humanities and Social Sciences
Humans
Keratoacanthoma - virology
Keratosis, Actinic - virology
Klinisk medicin
Lesions
Medical and Health Sciences
Medicin och hälsovetenskap
Medicinska och farmaceutiska grundvetenskaper
Metagenome
Microbiology in the medical area
Mikrobiologi inom det medicinska området
multidisciplinary
Other Clinical Medicine
Papillomaviridae
Phylogeny
Polymerase chain reaction
Science
Sequence Analysis, DNA
Skin - virology
Skin diseases
Skin Neoplasms - virology
Vaccines
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Description
Summary:Most viruses in human skin are known to be human papillomaviruses (HPVs). Previous sequencing of skin samples has identified 273 different cutaneous HPV types, including 47 previously unknown types. In the present study, we wished to extend prior studies using deeper sequencing. This deeper sequencing without prior PCR of a pool of 142 whole genome amplified skin lesions identified 23 known HPV types, 3 novel putative HPV types and 4 non-HPV viruses. The complete sequence was obtained for one of the known putative types and almost the complete sequence was obtained for one of the novel putative types. In addition, sequencing of amplimers from HPV consensus PCR of 326 skin lesions detected 385 different HPV types, including 226 previously unknown putative types. In conclusion, metagenomic deep sequencing of human skin samples identified no less than 396 different HPV types in human skin, out of which 229 putative HPV types were previously unknown.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep05807