The effect of adjunctive chlorhexidine mouthrinse on GCF MMP-8 and TIMP-1 levels in gingivitis: a randomized placebo-controlled study

• Published in: BMC oral health 2014-05, Vol.14 (1), p.55-55, Article 55
• Main Authors: Türkoğlu, Oya, Becerik, Sema, Tervahartiala, Taina, Sorsa, Timo, Atilla, Gül, Emingil, Gülnur
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Anti-Infective Agents, Local - therapeutic use; Antibiotics; Chlorhexidine - therapeutic use; Clinical trials; Dental Calculus - classification; Dental Plaque - complications; Dental Plaque - prevention & control; Dental Plaque Index; Dentistry; Disease; Double-Blind Method; Female; Females; Follow-Up Studies; Gingival Crevicular Fluid - drug effects; Gingival Crevicular Fluid - enzymology; Gingivitis - enzymology; Gingivitis - prevention & control; Health aspects; Hospitals; Humans; Male; Matrix Metalloproteinase 8 - drug effects; Medical research; Medicin och hälsovetenskap; Medicine, Experimental; Middle Aged; Mouthwashes - therapeutic use; Oral Hygiene Index; Periodontal Index; Periodontal Pocket - classification; Periodontal Pocket - prevention & control; Placebos; Tissue Inhibitor of Metalloproteinase-1 - drug effects; Young Adult
• ISSN: 1472-6831; 1472-6831
• DOI: 10.1186/1472-6831-14-55

The aim of the present study was to evaluate the effect of adjunctive chlorhexidine (CHX) mouthrinse on gingival crevicular fluid (GCF) MMP-8 and TIMP-1 levels in plaque-associated gingivitis. A total of 50 gingivitis patients were included in the present study. In addition to daily plaque control, CHX group rinsed with CHX, while placebo group rinsed with placebo mouthrinse for 4 weeks. GCF samples were collected, and clinical parameters including plaque index, papillary bleeding index, calculus index and pocket depth were recorded at baseline and 4 weeks. GCF MMP-8 and TIMP-1 levels were determined by immunofluorometric assay (IFMA) and enzyme-linked immunosorbent assay (ELISA), respectively. In both groups, GCF MMP-8 levels of anterior and posterior sites at four weeks were not different from baseline (p > 0.05). There were no significant differences in GCF MMP-8 levels between the study groups at four weeks (p > 0.05). GCF TIMP-1 levels of anterior and posterior sites at four weeks were higher compared to baseline in both groups (p  0.05). CHX usage had no significant effects on the GCF MMP-8 and TIMP-1 levels in plaque-associate gingivitis. However, daily plaque control resulted in the increase of GCF TIMP-1 levels regardless of CHX usage.