Contribution of allelic variability in prostate specific antigen (PSA) & androgen receptor (AR) genes to serum PSA levels in men with prostate cancer

• Published in: Indian journal of medical research (New Delhi, India : 1994) India : 1994), 2014-03, Vol.139 (3), p.371-378
• Main Authors: Chavan, Sushant V, Maitra, Anurupa, Roy, Nobhojit, Chavan, Padma R
• Format: Article
• Language: English
• Subjects: Alleles; Base Sequence; Genetic Variation - genetics; Genotype; Humans; Male; Microsatellite Repeats - genetics; Molecular Sequence Data; Original; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide - genetics; Promoter Regions, Genetic - genetics; Prostate-Specific Antigen - blood; Prostate-Specific Antigen - genetics; Prostatic Neoplasms - genetics; Receptors, Androgen - genetics; Sequence Analysis, DNA
• ISSN: 0971-5916; 0975-9174

Wide variability in serum prostate specific antigen (PSA) levels exists in malignant conditions of the prostate. PSA is expressed in normal range in 20 to 25 per cent of prostate cancer cases even in presence of high grade Gleason score. This study was aimed to assess the influence of genetic variants exhibited by PSA and androgen receptor (AR) genes towards the variable expression of PSA in prostate cancer. Pre-treatment serum PSA levels from 101 prostate cancer cases were retrieved from medical record. PSA genotype analysis in promoter region and AR gene microsatellite Cytosine/Adenine/Guanine (CAG) repeat analysis in exon 1 region was performed using DNA sequencing and fragment analysis techniques. A total of seven single nucleotide polymorphisms (SNPs) in the PSA promoter region were noted. Only two SNPs viz., 158G/A (P