Transplantation of Autologous Minced Bladder Mucosa for a One-Step Reconstruction of a Tissue Engineered Bladder Conduit

Surgical intervention is sometimes needed to create a conduit from the abdominal wall to the bladder for self-catheterization. We developed a method for tissue engineering a conduit for bladder emptying without in vitro cell culturing as a one-step procedure. In a porcine animal model bladder, wall...

Full description

Saved in:
Bibliographic Details
Published in:BioMed research international 2013-01, Vol.2013 (2013), p.1-10
Main Authors: Nordenskjöld, Agneta, Chamorro, Clara Ibel, Lundberg, Johan, Reinfeldt Engberg, Gisela, Fossum, Magdalena
Format: Article
Language:English
Subjects:
Abdomen
Abdominal wall
Animals
Biomedical research
Bladder
Bladder diseases
Care and treatment
Catheterization
Children & youth
Female
Health aspects
Hogs
Image Processing, Computer-Assisted
Intubation
Mucous Membrane - transplantation
Ostomy
Pediatrics
Plastic Surgery Procedures - methods
Rodents
Skin
Staining and Labeling
Studies
Tissue engineering
Tissue Engineering - methods
Transplantation, Autologous
Urinary Bladder - pathology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Surgical intervention is sometimes needed to create a conduit from the abdominal wall to the bladder for self-catheterization. We developed a method for tissue engineering a conduit for bladder emptying without in vitro cell culturing as a one-step procedure. In a porcine animal model bladder, wall tissue was excised and the mucosa was minced to small particles. The particles were attached to a tube in a 1 : 3 expansion rate with fibrin glue and transplanted back by attaching the tube to the bladder and through the abdominal wall. Sham served as controls. After 4-5 weeks, conduits were assessed in respect to macroscopic and microscopic appearance in 6 pigs. Two pigs underwent radiology before termination. Gross examination revealed a patent conduit with an opening to the bladder. Histology and immunostaining showed a multilayered transitional uroepithelium in all cases. Up to 89% of the luminal surface area was neoepithelialized but with a loose attachment to the submucosa. No epithelium was found in control animals. CT imaging revealed a patent channel that could be used for filling and emptying the bladder. Animals that experienced surgical complications did not form conduits. Minced autologous bladder mucosa can be transplanted around a tubular mold to create a conduit to the urinary bladder without in vitro culturing.
ISSN:2314-6133
2314-6141
DOI:10.1155/2013/212734