Matrix Metalloproteinase (MMP)‐8 and Tissue Inhibitor of MMP‐1 (TIMP‐1) Gene Polymorphisms in Generalized Aggressive Periodontitis: Gingival Crevicular Fluid MMP‐8 and TIMP‐1 Levels and Outcome of Periodontal Therapy

• Published in: Journal of periodontology (1970) 2014-08, Vol.85 (8), p.1070-1080
• Main Authors: Emingil, Gülnur, Han, Buket, Gürkan, Ali, Berdeli, Afig, Tervahartiala, Taina, Salo, Tuula, Pussinen, Pirkko J., Köse, Timur, Atilla, Gül, Sorsa, Timo
• Format: Article
• Language: English
• Subjects: Adenine; Adolescent; Adult; Aggressive periodontitis; Aggressive Periodontitis - enzymology; Aggressive Periodontitis - genetics; Aggressive Periodontitis - therapy; Alveolar Bone Loss - enzymology; Alveolar Bone Loss - therapy; Biomarkers - analysis; Cytosine; Dentistry; Female; Follow-Up Studies; Gene Frequency - genetics; genes; Genetic Predisposition to Disease - genetics; Genetic Variation - genetics; Genotype; gingival crevicular fluid; Gingival Crevicular Fluid - enzymology; Guanine; Humans; Male; matrix metalloproteinase 8; Matrix Metalloproteinase 8 - genetics; Medicin och hälsovetenskap; Middle Aged; Periodontal Attachment Loss - enzymology; Periodontal Attachment Loss - therapy; polymorphism; Polymorphism, Single Nucleotide - genetics; Sex Factors; Thymidine; Tissue Inhibitor of Metalloproteinase-1 - genetics; tissue inhibitor of metalloproteinase‐1; Treatment Outcome; Young Adult
• ISSN: 0022-3492; 1943-3670; 1943-3670
• DOI: 10.1902/jop.2013.130365

Background: The aim of the present study is to investigate matrix metalloproteinase (MMP)‐8 and tissue inhibitor of MMP‐1 (TIMP‐1) gene polymorphisms in generalized aggressive periodontitis (GAgP) and to assess the effects of MMP‐8 and TIMP‐1 genotypes on the outcomes of non‐surgical periodontal therapy. Methods: Genomic DNA was obtained from peripheral blood of 100 patients with GAgP and 167 periodontally healthy controls. MMP‐8 +17 C/G, −799 C/T, −381 A/G and TIMP‐1 372 T/C, *429 T/G polymorphisms were determined by polymerase chain reaction‐restriction fragment length polymorphism. Patients with GAgP received non‐surgical periodontal therapy and were followed for 6 months. Clinical periodontal parameters and gingival crevicular fluid (GCF) samples were collected at baseline and at follow‐up visits. GCF biomarkers were analyzed by immunofluorescence assay and enzyme‐linked immunosorbent assay. Results: Distribution of the MMP‐8 −799 C/T genotypes was significantly different between the GAgP and control groups (P