Effect of Transferrin Glycation on the Use of Carbohydrate-Deficient Transferrin as an Alcohol Biomarker

Measurement of an alcohol-induced shift in the serum transferrin glycosylation pattern, termed carbohydrate-deficient transferrin (CDT), is used as a biomarker for sustained high alcohol consumption. The present work examined whether non-enzymatic reaction of transferrin with glucose (glycation) mig...

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Bibliographic Details
Published in:Alcohol and alcoholism (Oxford) 2013-07, Vol.48 (4), p.478-482
Main Authors: HELANDER, Anders, MODEN, Naama Kenan
Format: Article
Language:English
Subjects:
Alcohol consumption
Alcohol Drinking - blood
Alcoholism and acute alcohol poisoning
Biological and medical sciences
Biological markers
Biomarkers - blood
Diabetes Mellitus - blood
Diabetics
Endokrinologi och diabetes
Farmakologi och toxikologi
Glucose
Glycerophospholipids - blood
Glycosylation
Klinisk medicin
Medical sciences
Medicin och hälsovetenskap
Medicinska och farmaceutiska grundvetenskaper
Quantification
Serum
Sialoglycoproteins - blood
Toxicology
Transferrin - analogs & derivatives
Transferrin - metabolism
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Summary:Measurement of an alcohol-induced shift in the serum transferrin glycosylation pattern, termed carbohydrate-deficient transferrin (CDT), is used as a biomarker for sustained high alcohol consumption. The present work examined whether non-enzymatic reaction of transferrin with glucose (glycation) might interfere with the use of CDT as an alcohol biomarker. The blood specimens were leftover volumes from the routine sample pool. Plasma and serum were collected among samples submitted for hemoglobin A1c (HbA1c) and CDT testing. Quantification of individual transferrin glycoforms in percentage of total transferrin was performed by an HPLC candidate CDT reference method. Incubating serum spiked with 20 or 200 mmol/l glucose caused time- and dose-dependent changes in the chromatographic profile of transferrin glycoforms, resulting in gradually wider peaks and reduced relative amounts of disialo- and trisialotransferrin. No similar chromatographic effects were seen in samples collected from diabetic patients with elevated HbA1c (>68 mmol/mol) values. These samples instead showed slightly higher mean %disialotransferrin levels (1.21%) compared with low HbA1c (
ISSN:0735-0414
1464-3502
1464-3502
DOI:10.1093/alcalc/agt045