Is a decrease of microparticles related to improvement of hemostasis after FVIII injection in hemophilia A patients treated on demand?

• Published in: Journal of thrombosis and haemostasis 2013-04, Vol.11 (4), p.697-703
• Main Authors: Mobarrez, F., Mikovic, D., Antovic, A., Antovic, J. P.
• Format: Article
• Language: English
• Subjects: cell‐derived microparticles; Factor VIII - administration & dosage; Factor VIII - pharmacology; Factor VIII - therapeutic use; FVIII; global hemostatic assay; Hematologi; hemophilia A; Hemophilia A - drug therapy; Hemostasis - drug effects; Humans; Klinisk medicin; Medicin och hälsovetenskap; Microspheres; Permeability
• ISSN: 1538-7933; 1538-7836; 1538-7836
• DOI: 10.1111/jth.12103

Summary Background Microparticles (MPs) are small membrane vesicles (0.1–1 μm) released from various cells after activation and/or apoptosis. There are limited data about their role in hemophilia A. Patients and Methods Blood samples were taken before and 30 min after FVIII injection in 18 patients with severe hemophilia A treated on demand. Flow‐cytometric determination of total MPs (TMPs) using lactadherin, platelet MPs (PMPs) (CD42a), endothelial MPs (EMPs) (CD144) and leukocyte MPs (LMPs) (CD45) was performed. The results were compared with data on endogenous thrombin potential (ETP), overall hemostatic potential (OHP), fibrin gel permeability and thrombin‐activatable fibrinolysis inhibitor (TAFI). Results and Conclusions TMPs and PMPs decreased after treatment (to 1015 ± 221 [SEM] and 602 ± 134 × 106 L−1) in comparison with values before treatment (2373 ± 618 and 1316 ± 331; P