Differentiation of mast cell subpopulations from mouse embryonic stem cells

Mast cells can generally be divided into two major groups, connective tissue mast cells and mucosal mast cells. We and others have previously shown that these mast cell populations can be developed in vitro from mouse bone marrow stem cells using a combination of specific growth factors and cytokine...

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Bibliographic Details
Published in:Journal of immunological methods 2012-08, Vol.382 (1-2), p.160-166
Main Authors: Westerberg, Christine Möller, Ullerås, Erik, Nilsson, Gunnar
Format: Article
Language:English
Subjects:
Animals
bone marrow
Cell Count
cell differentiation
Cell Differentiation - immunology
Cell Survival - immunology
Cells, Cultured
Connective
cytosolic diffusion
Embryonic stem cell
embryonic stem cells
Embryonic Stem Cells - cytology
Embryonic Stem Cells - immunology
IgE
interleukin-3
interleukin-4
interleukin-9
laboratory animals
Mast cell
mast cells
Mast Cells - cytology
Mast Cells - immunology
Mice
Mucosa
Peptide Hydrolases - analysis
Peptide Hydrolases - metabolism
phenotype
photoactivated localization microscopy
Receptors, Cell Surface - genetics
Receptors, Cell Surface - immunology
SCF
single particle tracking
stroboscopic illumination
transforming growth factor beta
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Summary:Mast cells can generally be divided into two major groups, connective tissue mast cells and mucosal mast cells. We and others have previously shown that these mast cell populations can be developed in vitro from mouse bone marrow stem cells using a combination of specific growth factors and cytokines. Mast cell differentiation from mouse embryonic stem (ES) cells is an important alternative method when developing mast cells from an embryonic lethal genetic deficiency or to reduce the use and handling of experimental animals. In this study, we have used protocols prior known to induce connective tissue like mast cells (CTLMC) (SCF and IL-4) and mucosal like mast cells (MLMC) (SCF, IL-3, IL-9 and TGF-β) from mouse bone marrow progenitor cells and employed these protocols to study if phenotype specific mast cells can be developed from ES cells. We here demonstrate that mast cells of the different phenotypes, CTLMC and MLMC, can be derived from mouse ES cells. The mast cell populations were characterized by chymase expression, receptor expression and their difference in activation pattern and in activation-induced survival.
ISSN:0022-1759
1872-7905
1872-7905
DOI:10.1016/j.jim.2012.05.020