Structural studies and biosynthetic aspects of the O-antigen polysaccharide from Escherichia coli O174

The structure of the repeating unit of the O-antigenic polysaccharide (PS) from Escherichia coli O174 has been determined. Component analysis together with 1H and 13C NMR spectroscopy experiments were employed to elucidate the structure. Inter-residue correlations were determined by 1H,13C-heteronuc...

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Bibliographic Details
Published in:Carbohydrate research 2012-06, Vol.354, p.102-105
Main Authors: Fontana, Carolina, Lundborg, Magnus, Weintraub, Andrej, Widmalm, Göran
Format: Article
Language:English
Subjects:
Biological repeating unit
Carbohydrate Conformation
carbon
Escherichia coli
Escherichia coli - chemistry
Escherichia coli - growth & development
Lipopolysaccharide
Magnetic Resonance Spectroscopy
Medicin och hälsovetenskap
NMR
nuclear magnetic resonance spectroscopy
O Antigens - biosynthesis
O Antigens - chemistry
Organic Chemistry
organisk kemi
polysaccharides
Reference Standards
stable isotopes
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Summary:The structure of the repeating unit of the O-antigenic polysaccharide (PS) from Escherichia coli O174 has been determined. Component analysis together with 1H and 13C NMR spectroscopy experiments were employed to elucidate the structure. Inter-residue correlations were determined by 1H,13C-heteronuclear multiple-bond correlation and 1H,1H-NOESY experiments. The PS is composed of tetrasaccharide repeating units with the following structure: [Display omitted] Cross-peaks of low intensity were present in the NMR spectra consistent with a β-d-GlcpNAc-(1→2)-β-d-GlcpA(1→ structural element at the terminal part of the polysaccharide, which on average is composed of ∼15 repeating units. Consequently the biological repeating unit has a 3-substituted N-acetyl-d-galactosamine residue at its reducing end.
ISSN:0008-6215
1873-426X
1873-426X
DOI:10.1016/j.carres.2012.02.020