Cost-effective HLA-Cw06:02 typing in a Caucasian population

:  Considering the clinical and genetic heterogeneity of psoriasis which may translate into distinct disease pathology and treatment response, correct typing of the main candidate gene HLA‐C is critical but not trivial. To facilitate genotyping, we compared established techniques with our newly deve...

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Bibliographic Details
Published in:Experimental dermatology 2012-03, Vol.21 (3), p.221-223
Main Authors: Nikamo, Pernilla, Ståhle, Mona
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Case-Control Studies
Cost-Benefit Analysis
Dermatology
European Continental Ancestry Group - genetics
Genetic Testing - economics
Genetic Testing - methods
genetics
Genotype
Genotypes
Genotyping
Histocompatibility antigen HLA
HLA
HLA-C Antigens - genetics
Humans
Medical sciences
Medicin och hälsovetenskap
Nucleotides
Polymorphism, Single Nucleotide - genetics
Psoriasis
Psoriasis - ethnology
Psoriasis - genetics
Psoriasis. Parapsoriasis. Lichen
Reproducibility of Results
Single-nucleotide polymorphism
SNP
Tissue typing
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Summary::  Considering the clinical and genetic heterogeneity of psoriasis which may translate into distinct disease pathology and treatment response, correct typing of the main candidate gene HLA‐C is critical but not trivial. To facilitate genotyping, we compared established techniques with our newly developed tool. Here, we propose that typing of four single nucleotide polymorphic markers within the HLA‐C region correctly determines HLA‐Cw*06:02 genotypes in psoriatic cases and healthy controls in a population of Caucasian origin. Typing of the SNPs presented herein proved to be precise, reliable, time and cost effective, and requiring low amount of DNA.
ISSN:0906-6705
1600-0625
1600-0625
DOI:10.1111/j.1600-0625.2011.01423.x