Hfq and Hfq-dependent small RNAs are major contributors to multicellular development in Salmonella enterica serovar Typhimurium

The RNA chaperone Hfq and its associated small RNAs (sRNAs) regulate a variety of phenotypes in bacteria. In this work, we show that Hfq is a master regulator of biofilm formation in Salmonella enterica serovar Typhimurium. Hfq and two Hfq-dependent sRNAs (ArcZ and SdsR) are required for rdar morpho...

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Bibliographic Details
Published in:RNA biology 2012-04, Vol.9 (4), p.489-502
Main Authors: Monteiro, Claudia, Papenfort, Kai, Hentrich, Karina, Ahmad, Irfan, Le Guyon, Soazig, Reimann, Romy, Grantcharova, Nina, Römling, Ute
Format: Article
Language:English
Subjects:
5' Untranslated Regions
ArcZ
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacterial Proteins - physiology
Binding
Biofilms
Biology
Bioscience
Calcium
Cancer
Cell
CsgD
curli fimbriae
Cycle
Fimbriae, Bacterial - genetics
Fimbriae, Bacterial - metabolism
Fimbriae, Bacterial - physiology
flagella
Gene Expression
Gene Expression Regulation, Bacterial
Landes
Medicin och hälsovetenskap
Molecular Chaperones - genetics
Molecular Chaperones - metabolism
Molecular Chaperones - physiology
Organogenesis
Phenotype
Proteins
RNA, Small Untranslated
RyeB
Salmonella typhimurium - genetics
Salmonella typhimurium - metabolism
Salmonella typhimurium - physiology
SdsR
type 1 fimbriae
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Summary:The RNA chaperone Hfq and its associated small RNAs (sRNAs) regulate a variety of phenotypes in bacteria. In this work, we show that Hfq is a master regulator of biofilm formation in Salmonella enterica serovar Typhimurium. Hfq and two Hfq-dependent sRNAs (ArcZ and SdsR) are required for rdar morphotype expression in S. typhimurium. Hfq controls rdar biofilm formation through the major biofilm regulator CsgD. While csgD mRNA steady-state levels are altered in a sdsR mutant, ArcZ seems to work mainly at the post-transcriptional level. Overexpression of ArcZ complemented rdar morphotype formation of an hfq mutant under plate-grown conditions. Although ArcZ activates rpoS expression, its effect on csgD expression is mainly independent of RpoS. ArcZ does not only regulate rdar morphotype expression, but also the transition between sessility and motility and the timing of type 1 fimbriae vs. curli fimbriae surface-attachment at ambient temperature. Consequently, ArcZ is a major regulator of rdar biofilm development.
ISSN:1547-6286
1555-8584
1555-8584
DOI:10.4161/rna.19682